-
Plasmid#184711PurposePKA biosensorDepositorInsertBooster-PKA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-iRFP670-P2Av3-RGECO1.0
Plasmid#184713PurposeCalcium biosensorDepositorInsertGCaMP6s
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-AcrAB-AsLOV2*(543)
Plasmid#210863PurposeIPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.DepositorInsertsacrA
acrB
UseSynthetic BiologyTagsAsLOV2*(543)ExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xABRE_R
Plasmid#185804Purposea synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the RD29A promoterDepositorInsert6xABRE(RD29A)+-86MP+erGFP
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FB401
Plasmid#209130PurposeMultipartite assembly of the TU for afpA expression under the regulation of afpA promoter and terminator with the pyr4 auxotrophy marker pyr4 from T. reseeiDepositorInsertPafpA:afpA:TafpA::Ppyr4:pyr4:Tpyr4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep
Plasmid#208644Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
UseCRISPR; Ssdna donnor preparationTagsTwin StrepExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNGsacB
Plasmid#203676PurposepQFT with mNeonGreen and sacB under control of PQJDepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQJAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0006
Plasmid#205575PurposeGlucos(1-231)-NpuDnaE(N)DepositorInsertsGlucosyltransferase and NpuDnaE(N)_Arabidopsis-codon-OP
CYP76AD1-P2A-DODA
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrc-CysGA-Im7 F84A
Plasmid#201082PurposeIm7 F84A variant inserted into CysGA at G364 via GS linkerDepositorInsertCysGA-Im7 F84A
UseTagsExpressionBacterialMutationIm7 F84APromoterpTrcAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pV1F(Spec)
Plasmid#205086PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag Hygro BFP nLAP +0
Plasmid#194317PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertHygro-P2A-nLAP(BFP)
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF_TypeIC_target_sfGFP_invivo
Plasmid#196399Purposeexpresses CRISPR Cascade Type IC target sequence for in vivo assayDepositorInserttype IC dsDNA target
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dCas9-KRAB-gRNA-TRE-blast
Plasmid#201152PurposeLentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsdCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAExpressionMutationgRNA sequence: TACGTTCTCTATCACTGATPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
XLone-mARG2
Plasmid#173792PurposeMammalian acoustic reporter gene 1 cassette 2 on XLone plasmidDepositorInsertGvpN, F G L S K J U
UseTagsGFPExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-UBC-mARG3
Plasmid#173793PurposeMammalian acoustic reporter gene 1 cassette 3 on PB-UBC plasmidDepositorInsertGvpJ, F, G, L, K
UseTagsExpressionMammalianMutationPromoterUBC promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg20-24 pST39
Plasmid#200439PurposeHis6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.DepositorInsertsAtg20
Atg24
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHACK-QF2-AgU6-mCherry
Plasmid#184530PurposeHACK-compatible plasmid for generating T2A-QF2 knockins in Anopheles mosquitoes with a mCherry selectable marker.DepositorInsertQF2
UseCRISPRTagsExpressionMutationPromoterAnopheles U6 promoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP)
Plasmid#172339PurposemnucTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)TetR
Plasmid#172352PurposeTet Repressor reporterDepositorInsertsTetR
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2_claudink:Cas9green; erbb2_gRNA171
Plasmid#199339PurposepDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNADepositorInsertsclaudink
Cas9
erbb2 sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only