pUC-ODN-hsRPL3-CterTwStrep
(Plasmid
#208644)
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Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208644 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC-ODN
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Modifications to backbonerefer to (Addgene#208642)
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Vector typeCRISPR ; ssDNA donnor preparation
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTwin Strep tag with RPL3 genome HomoArm
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Alt nameDonnor DNA
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SpeciesH. sapiens (human), Synthetic
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GenBank ID5'HomoArm: NC_000022.11:39313122~39312943 3'HomoArm: NC_000022.11:39312939~39312773
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Entrez GeneRPL3 (a.k.a. ASC-1, L3, TARBP-B, uL3)
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Tag
/ Fusion Protein
- Twin Strep
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
#important# This is a Intermediate plasmid, and WILL fail the CRISPR knock-in. Use Addgene#208645, do not use this plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC-ODN-hsRPL3-CterTwStrep was a gift from Quanfu Ma (Addgene plasmid # 208644 ; http://n2t.net/addgene:208644 ; RRID:Addgene_208644)
