Addgene

We narrowed to 17,147 results for: gateway

Showing: 11041 - 11060 of 17147 results

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pLenti PGK Neo DEST (w531-1)

Plasmid

#19067

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Scot Wolfe, Rene Maehr

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

Plasmid

#118617

Purpose

Lentiviral expression of mTurq2 tagged PCNA

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

TK-NLS-mTurq2-PCNA (PCNA Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA_DENV2-NS2B3_V5

Plasmid

#115906

Purpose

Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

Depositor

Alan Rothman

Article

Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

Insert

DENV2 NS2B3 protease

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Hygro DEST (693-2)

Plasmid

#17291

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_MYC

Plasmid

#167715

Purpose

Expresses MYC in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

MYC (MYC Human)

Use

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-wtSrc

Plasmid

#140294

Purpose

Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pT3-EF1α-Dest

Plasmid

#133299

Purpose

The pT3-EF1α destination vector without any insert which can be used for LR reaction.

Depositor

Xin Chen

Article

Lee et al Hepatology. 2008 Apr;47(4):1200-10. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarR2

Plasmid

#112813

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdTomato

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6/UbC/mSlc7a1

Plasmid

#17224

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Slc7a1 (Slc7a1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]

Plasmid

#159900

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mcherry | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Pmlc-1

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG2

Plasmid

#112814

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdGFP

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-C

Plasmid

#118372

Purpose

Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAD/CMV/V5-DEST-Cdc42-2G

Plasmid

#68812

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Adenoviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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p8327 pLIX YAP1 S127A

Plasmid

#184528

Purpose

Expression of YAP1 S127A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S127A (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S127A

Promoter

TRE promoter, Tet ON

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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RIPK4_pCSdest

Plasmid

#53897

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RIPK4 (RIPK4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pGFP-Rab8A[Q67L]

Plasmid

#24900

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[Q67L]

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Glutamine 67 to Leucine

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-wtSrc

Plasmid

#140295

Purpose

Expresses Flag-tagged wild-type human Src in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src wildtype (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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Ubi:Zebrabow

Plasmid

#118219

Purpose

For ubiquitous expression of Brainbow in zebrafish

Depositor

Alex Schier, Albert Pan

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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TurboID-mNeongreen-3xFLAG fusion pos3

Plasmid

#172864

Purpose

C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

Depositor

Mario de Bono

Article

Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

Insert

TurboID

Use

Unspecified

Tags

FLAG, mNeongreen, and myc

Expression

Mutation

Promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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