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C453-E21: attB4-FerHp>-attB5

Plasmid

#162978

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ferritin heavy chain promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJC8

Plasmid

#170757

Purpose

Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.

Depositor

Roberto Dominguez

Article

Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

Type

Empty backbone

Use

Tags

site 1- V5 tag and site 2- FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJH198_pE12_hyCal2-eGFP

Plasmid

#160411

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to eGFP

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyCal2::eGFP

Use

Other

Tags

eGFP

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH200_pE12_hyCal7-eGFP

Plasmid

#160413

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system fused to eGFP

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyCal7::eGFP

Use

Other

Tags

eGFP

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH201_pE12_hyCal7-mCherry

Plasmid

#160414

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system fused to mCherry

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyCal7::mCherry

Use

Other

Tags

mCherry

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH202_pE12_hyCal9-eGFP

Plasmid

#160415

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system fused to eGFP

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyCal9::eGFP

Use

Other

Tags

eGFP

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJH203_pE12_hyCal9-mCherry

Plasmid

#160416

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system fused to mCherry

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyCal9::mCherry

Use

Other

Tags

mCherry

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

Plasmid

#162936

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E01: attB5r-meGFP-nostop-attB1r

Plasmid

#162940

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

Plasmid

#162937

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6 fusion, AcMNPV polyhedrin promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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