Addgene

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SEPT11 sh2 shRNA

Plasmid

#122023

Purpose

Knock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRDA_103

Plasmid

#133460

Purpose

H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

control guide

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Zeo/pTER shLUC (w648-1)

Plasmid

#21561

Purpose

3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Zeo

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly luciferase shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pJG484

Plasmid

#91194

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi8+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSMP-NR2F1_2

Plasmid

#36363

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

NR2F1 (NR2F1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
beta4-nAChR (DPM negative control)

Plasmid

#86651

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNB4 (control)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMGC

Plasmid

#112812

Purpose

PCR template vector for amplifying gRNA(F+E)-OstRNA fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

gRNAcore(F+E)-tRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shPIK3CD.v2 puro

Plasmid

#58707

Purpose

Lentiviral shRNA vector for knockdown of human PIK3CD

Depositor

Kevin Janes

Article

Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Insert

shPIK3CD (PIK3CD Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only
Neurl1Rm-Flag pcDNA3.1

Plasmid

#17319

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pBS/U6/hTID1

Plasmid

#13745

Depositor

Karl Munger

Article

Edwards et al Oncogene. 2004 Nov 4. 23(52):8419-31.

Insert

hTid1 siRNA (DNAJA3 Human)

Use

RNAi

Tags

Expression

Mutation

Inverted repeat of 21 bp with 6 nuc. spacer.

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-shMePCE

Plasmid

#113536

Purpose

Retroviral vector designed to knock down MePCE.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE (MEPCE Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSMP-NR2F1_3

Plasmid

#36364

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

NR2F1 (NR2F1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pQCXIN X2/pTER shEGFP#1 (w491-1)

Plasmid

#19167

Purpose

Retroviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #2

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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NHR1+2-Flag pcDNA3.1

Plasmid

#17316

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3_3_TOPO_TA-ms2-VP64

Plasmid

#79371

Purpose

Recruits VP64 to a compatible gRNA for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-VP64

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-PM BRD7 sh3

Plasmid

#41930

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-PM BRD7 sh2

Plasmid

#41928

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pICSL11058

Plasmid

#68254

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_TaU6 + sgRNA_HvPM19_3

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSMP-NR2F1_1

Plasmid

#36362

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

NR2F1 (NR2F1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
NHR1-Flag pcDNA3.1

Plasmid

#17315

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only