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Plasmid#71743PurposeContains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activityDepositorInsertUncoupling Protein 3 promoter (Ucp3 Mouse)
UseTagsLuciferaseExpressionBacterialMutationContains 7kb of the murine UCP3 gene encompassing…PromoterUCP3Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot8-D40AE42A_AH
Plasmid#148902PurposeMammalian Expression of HsNot8-D40AE42ADepositorInsertHsNot8-D40AE42A (CNOT8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-ybbR
Plasmid#60866PurposeCharacteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tagDepositorInsertsCohIII
CBM
UseTags6xHIS, HRV 3C, and ybbRExpressionBacterialMutationCysteine 63 to Serine in CBM modulePromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HSPA5P
Plasmid#44150Purposea fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)DepositorInsertHSPA5 promoter (HSPA5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
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R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)
Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorInsertHis6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp13_GC3opt
Plasmid#157715Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBTet-SNAP-Cep78
Plasmid#136827PurposeMammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep78 (CEP78 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG
Plasmid#74261PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.Y591X truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-ALMS1
Plasmid#136828PurposeMammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tagDepositorInsertSNAP-ALMS1 (ALMS1 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sap-SH2
Plasmid#46477DepositorInsertSap (SH2D1A Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 4-128PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf5(WT)-mCherry
Plasmid#79421PurposeExpresses C-terminally mCherry-tagged Arf5(WT) in mammalian cellsDepositorInsertArf5 (ARF5 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-EF1.Pro
Plasmid#79488PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoterDepositorInsertEF1 Promoter (EEF1A1 Human, Synthetic)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterEF1 PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf3(WT)-mCherry
Plasmid#79420PurposeExpresses C-terminally mCherry-tagged Arf3(WT) in mammalian cellsDepositorInsertArf3 (ARF3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-358-eGFP
Plasmid#110693PurposeExpresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFPDepositorInsertCPSF6-eGFP (CPSF6 )
UseLentiviralTagsEGFPExpressionMutationTruncation of CPSF6 isoform 2 at amino acid 358PromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMG058 MBP-GS 634-666-His
Plasmid#154077PurposeExpresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coliDepositorInsertGYS1 (GYS1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LYN-1/1
Plasmid#91536PurposeProtein expression and purification of human SH3 domain construct LYN-1/1DepositorInsertLYN-1/1 (LYN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(WT)
Plasmid#27134DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-miR-124-sponge (pos. CTRL)
Plasmid#117327PurposeDual luciferase assay positive control for miR-124 bindingDepositorInsertmiR-124 sponge
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 D122A HA pCAGGS
Plasmid#206097Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationD122APromoterCMV/B-actinAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only