pGEX Sap-SH2
(Plasmid
#46477)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX6P1
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Backbone manufacturerGE Healthcare Biosciences
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSap
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Alt nameSH2D1A
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Alt nameLYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; FLJ18687; FLJ92177; SH2D1A
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SpeciesH. sapiens (human)
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Insert Size (bp)388
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Mutationcontains amino acids 4-128
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Entrez GeneSH2D1A (a.k.a. DSHP, EBVS, IMD5, LYP, MTCP1, SAP, SAP/SH2D1A, XLP, XLPD, XLPD1)
- Promoter Tac
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- PreScission (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The molecular weight of the GST fusion protein is approximately 38.9 kDa
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX Sap-SH2 was a gift from Bruce Mayer (Addgene plasmid # 46477 ; http://n2t.net/addgene:46477 ; RRID:Addgene_46477) -
For your References section:
High-throughput phosphotyrosine profiling using SH2 domains. Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, Saksela K, Schiller MR, Shin DG, Mayer BJ. Mol Cell. 2007 Jun 22;26(6):899-915. 10.1016/j.molcel.2007.05.031 PubMed 17588523