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Neuroplastin-CD4d3+4-bio

Plasmid

#73111

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Neuroplastin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Neuroplastin (NPTN Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pROSA26-Donor

Plasmid

#60952

Purpose

Porcine ROSA26 locus targeting

Depositor

Liangxue Lai

Article

Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

Insert

Porcine ROSA26 homologous ARM

Use

Gene targeting

Tags

Neo EGFP

Expression

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pFAB3677

Plasmid

#47833

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 2 and BCD1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-TUBA1B_sgRNA

Plasmid

#183889

Purpose

pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcw107

Plasmid

#64648

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pRetro-XT-PAK2-CFP(puro)-WT

Plasmid

#81176

Purpose

Retroviral vector for expression of wild-type PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Expression

Mutation

Promoter

P-tight TRE

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
tetO-TBX20

Plasmid

#170705

Purpose

doxycycline-inducible overexpression of TBX20

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

TBX20 (TBX20 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

Plasmid

#203439

Purpose

Mammalian expression of RUNX4

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

RUNX3 (RUNX3 Human)

Use

Tags

3 x FLAG

Expression

Mammalian

Mutation

R122C

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Plxna4-Fc-His

Plasmid

#72125

Purpose

Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna4 (Plxna4 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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tetO-SNAI2

Plasmid

#170702

Purpose

doxycycline-inducible overexpression of SNAI2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SNAI2 (SNAI2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPong_v2

Plasmid

#47101

Purpose

Expresses the Pong ORF1 and transposase from rice to allow mPing movement. Contains ORF1 and transposase driven by a native soybean promoter. Contains mPing and BAR for basta selection.

Depositor

Wayne Parrott

Article

Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

Inserts

mPing
Pong Open Reading Frame 1 (ORF1)
Pong Transposase

Use

Plant expression

Tags

Expression

Mutation

Promoter

GmUbi_AP, GmUbi_XS, and no promoter

Available since

Oct. 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

Plasmid

#136513

Purpose

Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

Depositor

Erik Sontheimer

Article

Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

Insert

codon-optimized AcrIIA5

Use

Tags

FLAG/NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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Arabidopsis thaliana Atm3 d80 pET21a+

Plasmid

#173045

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

with N-terminal 80 amino acids deletion

Promoter

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAT-mCherry2-G

Plasmid

#164169

Purpose

Expresses mCherry2 under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-SAV1

Plasmid

#166476

Purpose

Expresses fusion of mEGFP and SAV1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, SAV1 (SAV1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 3SA

Plasmid

#121490

Purpose

Expression of human PD-L1 3SA with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Inserts

PD-L1 3SA
humun PD-L1 shRNA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S176A, T180A, S184A

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pD765 FDFT1 3'UTR

Plasmid

#105765

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

FDFT1 3'UTR (FDFT1 Human)

Use

Luciferase

Tags

Expression

Mutation

None

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Intersectin I SH3 A domain (human)

Plasmid

#47413

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain A (ITSN1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain A

Promoter

tac

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pLIC-His-RGS4

Plasmid

#204847

Purpose

Express His tagged-RGS4 in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

RGS4 (RGS4 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTET2-lucCP

Plasmid

#198353

Purpose

Part of Renilla reporter system, encodes Firefly luciferase

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Firefly Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

pTET2

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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