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Plasmid#136800PurposeMammalian expression of the centrosomal protein Cep97 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep97 (CEP97 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BCAR1-1/1
Plasmid#91418PurposeProtein expression and purification of human SH3 domain construct BCAR1-1/1DepositorInsertBCAR1-1/1 (BCAR1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-K650E
Plasmid#191757PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)DepositorInsertmGFP-FGFR3IIIc
UseTagsmGFPExpressionMammalianMutationc.1948A>G (p.K650E) + silent mutation c.1938C&…PromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Bmx-SH2
Plasmid#46409DepositorInsertBmx (BMX Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 286-400PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag Leucyl tRNA synthetase pRK5
Plasmid#46341DepositorInsertleucyl tRNA synthetase (LARS1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-GAP43-ECFP-stop-L2
Plasmid#186356PurposeEntry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequencesDepositorInsertGrowth-associated protein-43 (Gap43 Mouse, Synthetic)
UseExpression of a fluorescent membrane markerTagsECFPExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-MVP delta607-623aa
Plasmid#204544PurposeExpresses EGFP-tagged MVP delta607-623aa in mammalian cellsDepositorInsertMVP (MVP Human)
UseTagsExpressionMammalianMutationdelta607-623aaPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-NpHR3.3-EYFP
Plasmid#137151PurposeViral expression of NpHR3.3-EYFPDepositorHas ServiceAAV8InsertNpHR3.3-EYFP
UseAAVTagsExpressionMutationW179FPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRCreb5gRNA
Plasmid#195021PurposeSequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domainDepositorInsertCreb5 gRNA (CREB5 Bovine)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4 fusion tools screening plasmid #1
Plasmid#174511PurposeCloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.DepositorInsert[BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nsp13 K289A-EGFP
Plasmid#165130Purposemammalian expression and localizationDepositorInsertNsp13 K289A (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
shBIRC5 # 2
Plasmid#42554DepositorInsertshBIRC5 (BIRC5 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(N)-SH2
Plasmid#46432DepositorInsertGap/RASGAP(N) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 168-283PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF073 MBP-Axin-His
Plasmid#154011PurposeExpresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coliDepositorInsertAxin1 (AXIN1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromotertacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(N)-SH2
Plasmid#46498DepositorInsertSHP-2(N) (PTPN11 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-104PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Nsp15
Plasmid#165120Purposemammalian expression and localizationDepositorInsertNsp15 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-K508M
Plasmid#191753PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)DepositorInsertmGFP-FGFR3IIIc
UseTagsmGFPExpressionMammalianMutationc.1523A>T (p.K508M) + silent mutation c.1515A&…PromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
shBIRC5 # 1
Plasmid#42553DepositorInsertshBIRC5 (BIRC5 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry+NLS
Plasmid#128013PurposeVector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4 DBD-SV40NLS-3x-Flag
UseGal4 entry vector with nls to generate tethering …TagsGal4-DBD-SV40NLS-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-Jun4A-Myc
Plasmid#47444Purposemammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)DepositorInsertJun 4A mutant (Jun Mouse)
UseTagsFlag and mycExpressionMammalianMutationfour N-terminal residues phosphorylated by JNK, s…PromoterCMVAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only