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Showing: 10981 - 11000 of 16204 results

  2. pHBJT034

    Plasmid
    #225180
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225180-plasmid-map-sequence-id-444744

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  3. pHBJT032

    Plasmid
    #225178
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (TetR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225178-plasmid-map-sequence-id-444695

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  4. pHBJT033

    Plasmid
    #225179
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (GmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225179-plasmid-map-sequence-id-444689

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  5. pHBJT030

    Plasmid
    #225176
    Purpose
    Low copy cloning vector with mCherry integrated in the multiple cloning site (Chlr). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225176-plasmid-map-sequence-id-444686

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  6. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

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  7. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212121-plasmid-map-sequence-id-444186

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  12. Tol2-U6.3-sgRNA-empty-GFP

    Plasmid
    #221843
    Purpose
    empty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Insert
    EGFP
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221843-plasmid-map-sequence-id-437471

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  13. Tol2-U6.3-sgRNA-DNM1L -GFP

    Plasmid
    #221845
    Purpose
    Tol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Inserts
    EGFP
    DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221845-plasmid-map-sequence-id-437474

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  14. Tol2-U6.3-sgRNA-MFN1-GFP

    Plasmid
    #221846
    Purpose
    Tol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promote
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Inserts
    EGFP
    MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221846-plasmid-map-sequence-id-437473

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  15. PYPQ121-DdCBE

    Plasmid
    #218174
    Purpose
    Express plastid DdCBE, composed of TALE, split DddA originated from Burkholderia cenocepacia and UGIs
    Depositor
    Yiping Qi
    Article
    Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.
    Insert
    TALE-Intron-N-DddAtox half (G1397N)-UGI-T2A-TALE-C-DddAtox half (G1397C)-UGI
    Use
    Plastid base editing
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    218174-plasmid-map-sequence-id-437206

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  17. pQC

    Plasmid
    #221134
    Purpose
    Contains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminator
    Depositor
    Daniel Daley
    Article
    Cumming et al ACS Synth Biol. 2024 Apr 27. doi: 10.102
    Inserts
    [PibpA-gfpASV]
    [PcpxPmcherryASV] OPT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cpxP and ibpA
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221134-plasmid-map-sequence-id-437038

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  19. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid
    #184820
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.
    Depositor
    Marnie Halpern
    Article
    Spikol et al eLife 12:RP89516
    Insert
    rln3a-HA
    Use
    CRISPR; For integration of qf2 by homology-direct…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    184820-plasmid-map-sequence-id-366119

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  20. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid
    #208625
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 72~197, other residues not included
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208625-plasmid-map-sequence-id-433638

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Showing: 10981 - 11000 of 16204 results