pET28-RPL3-72~197-mEGFP(A206K)-TwStrep
(Plasmid
#208625)
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Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208625 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28-mEGFP(A206K)-TwStrep
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Modifications to backbonerefer to (Addgene#208622)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionswe use BL21 (DE3) for expression
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehuman RPL3 (ENST00000216146) aa 72~197
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Alt nameuL3
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Alt nameRPL3
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Alt nameribosomal large unit protein L3
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SpeciesH. sapiens (human)
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Mutationonly aa 72~197, other residues not included
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GenBank IDENST00000216146
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Entrez GeneRPL3 (a.k.a. ASC-1, L3, TARBP-B, uL3)
- Promoter T7
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Tags
/ Fusion Proteins
- Twin Strep (C terminal on backbone)
- 6xHis (N terminal on backbone)
- mEGFP(A206K) (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pETupstream, T7p
- 3′ sequencing primer T7ter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-RPL3-72~197-mEGFP(A206K)-TwStrep was a gift from Quanfu Ma (Addgene plasmid # 208625 ; http://n2t.net/addgene:208625 ; RRID:Addgene_208625)