-
Plasmid#184887PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pECNUS3
Plasmid#184886PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECNUS1
Plasmid#184884PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRD503
Plasmid#220788PurposeOverexpresses HTkC in E. coli cellsDepositorInsertHTkC
UseTagsExpressionBacterialMutationPromoterT7 promotorAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRD551
Plasmid#220789PurposeOverexpresses HMfC in E. coli cellsDepositorInsertHMfC
UseTagsExpressionBacterialMutationPromoterT7 promotorAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFHMSPN-avi-TEV-LIC V
Plasmid#220885PurposeDonor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.DepositorTypeEmpty backboneUseBaculovirus expresssionTagsHoneybee melittin signal followed by His, avi and…ExpressionMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-iGABASnFR2n-WPRE
Plasmid#218884PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAVTagsExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-iGABASnFR2-WPRE
Plasmid#218883PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAVTagsExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_BA.2.87.1
Plasmid#218679Purposeexpressing SARS-CoV-2 BA.2.87.1 spike protein for pseudovirus productionDepositorInsertSpike (BA.2.87.1)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
Plasmid#217970PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.DepositorInsert2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK7-g7g8-K8
Plasmid#218220PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK3-g3g4-K4
Plasmid#218218PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK5-g5g6-K6
Plasmid#218219PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Prepro-3x-Adan
Plasmid#181733PurposeGalactose inducible expression of Prepro-3x-AdanDepositorInsertPrepro-3x-Adan
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vMAT1-VL-hIgG1-CL
Plasmid#218441Purposemammalian expression plasmid for the light chain fragment of an anti-vMAT1 Fab domainDepositorInsertvMAT1-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vGLUT1-VL-hIgG1-CL
Plasmid#218439Purposemammalian expression plasmid for the light chain fragment of an anti-vGLUT1 Fab domainDepositorInsertvGLUT1-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vMAT2-VL-hIgG1-CL
Plasmid#218443Purposemammalian expression plasmid for the light chain fragment of an anti-vMAT2 Fab domainDepositorInsertvMAT2-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Kar2-Beta-Amyloid
Plasmid#181707PurposeGalactose inducible expression of Kar2-Beta-AmyloidDepositorInsertKar2-Beta-Amyloid
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-RNQ1
Plasmid#181706PurposeGalactose inducible expression of RNQ1DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXC4-AroPERFECT
Plasmid#215588PurposeFor overexpression of Gal4-DBD HOXC4 AroPERFECTDepositorInsertHOXC4 AroPERFECT (HOXC4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only