Purpose
Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
Insert
BACH2-sgRNA8 (
BACH2 Synthetic, Human, S. pyogenes)
Use
CRISPR
Tags
3xFLAG, SV40 NLS, and T2A-PuroR
Expression
Mammalian
Mutation
D10A and H840A in S.pyogenes Cas9; E756A inactiva…
Available since
March 7, 2016
Availability
Academic Institutions and Nonprofits only