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pPB-shCtnnd2.1-mCherry-CAAX

Plasmid

#209107

Purpose

Membrane tethered mCherry shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.1

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPB-scrCtnnd2.1-mCherry-CAAX

Plasmid

#209108

Purpose

Membrane tethered mCherry scramble of shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.1

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ePB-PURO-TT-PA-CRE

Plasmid

#179584

Purpose

ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

PA-MAG-CRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

Plasmid

#174610

Purpose

PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

human CD19, antigen minigene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AAV-short_BrokenHeart (no BC)

Plasmid

#86950

Purpose

AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

Depositor

Rob Mitra, Joseph Dougherty

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

Brokenheart construct

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-TAC-ERP2-(N-HA)KRAS_G12D

Plasmid

#201254

Purpose

Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

Depositor

Alexander Kleger

Article

Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

Insert

KRAS (KRAS Human)

Use

Piggybac transposon

Tags

HA-Tag

Expression

Mammalian

Mutation

changed Gly (G) 12 to Asp (D)

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2

Plasmid

#172115

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

Promoter

EF1a and TRE3G

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNIL

Plasmid

#172113

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

Depositor

Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
hISL1 (ISL1 Human)
hLHX3 (LHX3 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Mutation

Promoter

EF1a and TRE3G

Availability

Academic Institutions and Nonprofits only

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