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Plasmid#48354PurposeContributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.DepositorInsertIRES_ECFP
UseGateway entry vectorTagsExpressionMutationECFP translation is mediated by an IRES. Contains…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)
Plasmid#224497PurposeGateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)DepositorInsertActin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)
Plasmid#224496PurposeGateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)DepositorInsertActin-Vhh-sfGFP-P2A-HA-iRFP-caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/EV
Plasmid#120848PurposeControl vector, 20nt random sequence between gateway recombination sitesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR.hU6hH1
Plasmid#32686Purposedual shRNA expression cassette entry vectorDepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterhU6hH1Available sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-GFP-V5-TurboID_IDG-K
Plasmid#135241PurposeGateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertGFP-V5-TurboID
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E005 Hs.AKT3
Plasmid#70289PurposeGateway ORF clone of human AKT3 [NM_005465.4] with stop codon (for native or N-terminal fusions)DepositorInsertAKT 3 (AKT3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TMPRSS2
Plasmid#158441PurposeGateway Cloning compatible entry vector for the human TMPRSS2 gene.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse-Dysferlin-Transcript-Variant1
Plasmid#60214PurposeMouse dysferlin transcript variant-1 (accession #NM_021469) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (Dysf Mouse)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-eGFP_IDG-K
Plasmid#135263PurposeGateway destination clone of eGFP (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-eGFP
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-TurboID-V5-eGFP_IDG-K
Plasmid#135264PurposeGateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTurboID-V5-eGFP
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-TurboID-V5-dsRed_IDG-K
Plasmid#135261PurposeGateway destination clone of dsRed (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTurboID-V5-dsRed
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-GFP-V5-miniTurbo_IDG-K
Plasmid#135240PurposeGateway destination clone of GFP (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertGFP-V5-miniTurbo
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-hcRed-V5-miniTurbo_IDG-K
Plasmid#135237PurposeGateway destination clone of hcRed (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInserthcRed-V5-miniTurbo
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TMPRSS11D
Plasmid#158447PurposeGateway Cloning compatible entry vector for the human TMPRSS11D gene.DepositorInsertTMPRSS11D (TMPRSS11D Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E085 Hs.FGFR2
Plasmid#70369PurposeGateway ORF clone of human FGFR2 [NM_000141.4] with stop codon (for native or N-terminal fusions)DepositorInsertFGFR2 (FGFR2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse-Dysferlin-Transcript-Variant2
Plasmid#60215PurposeMouse dysferlin transcript variant-2 (accession #NM_001077694) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (Dysf Mouse)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human-Dysferlin-v1
Plasmid#60217PurposeHuman dysferlin exon 1 variant (accession #DQ267935) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (DYSF Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-BioID-STOP_IDG-K
Plasmid#158163PurposeGateway destination clone of BioID (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-BioID
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
R777-E101 Hs.HRAS
Plasmid#70385PurposeGateway ORF clone of human HRAS [NM_005343.2] with stop codon (for native or N-terminal fusions)DepositorInsertHRAS (HRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only