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eIF3a- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192235

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mClover-NeoR (EIF3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192243

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mClover-NeoR (EIF3K Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192244

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192237

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mClover-NeoR (EIF3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192238

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Toxoplasma gondii CBS-pair domain

Plasmid

#166438

Purpose

Express pGEX-6P-1 Toxoplasma gondii CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Toxoplasma gondii CBS-pair domain

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac, LacI, Amp

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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fru-T2A-CsChrimson-tdTomato HDR plasmid

Plasmid

#141100

Purpose

Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

Depositor

Leslie Vosshall

Article

Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

Insert

fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm

Use

Donor template

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Blasto_TtoC and hairpin extension

Plasmid

#167920

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 P290L BLAST

Plasmid

#164239

Purpose

lentiviral plasmid for GFP-TREX1 P290L

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

P290L point mutation

Promoter

CMV

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 Y305C BLAST

Plasmid

#164240

Purpose

lentiviral plasmid for GFP-TREX1 Y305C

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Y305C point mutation

Promoter

CMV

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 D272fs BLAST

Plasmid

#164237

Purpose

lentiviral plasmid for GFP-TREX1 D272fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D272 frame shift mutation

Promoter

CMV

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 L287fs BLAST

Plasmid

#164238

Purpose

lentiviral plasmid for GFP-TREX1 L287fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

L287 frame shift mutation

Promoter

CMV

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

Plasmid

#169743

Purpose

CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

mScarlet-I

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pCMV-tetO2

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165889

Purpose

Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

Plasmid

#173167

Purpose

Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Calcineurin Like EF-Hand Protein 1 (CHP1 Human)

Use

Retroviral

Tags

3XFLAG

Expression

Mutation

Synonymous mutations at sgRNA sites, G2A, S6A

Promoter

MMLV

Available since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDY156: SUZ12 px330 sgRNA Cas9 plasmid

Plasmid

#170792

Purpose

This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 309* BLAST

Plasmid

#164242

Purpose

lentiviral plasmid for GFP-TREX1 309*

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deletion of amino acids 310-314

Promoter

CMV

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 G306A BLAST

Plasmid

#164241

Purpose

lentiviral plasmid for GFP-TREX1 G306A

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

G306A point mutation

Promoter

CMV

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pJM457 EF1alpha EBFP2-P2A-BlastR (TUPV8)

Plasmid

#139237

Purpose

mMoClo TUPV8, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

Plasmid

#139236

Purpose

mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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