pGEX-6P-1 Toxoplasma gondii CBS-pair domain
(Plasmid
#166438)
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PurposeExpress pGEX-6P-1 Toxoplasma gondii CBS-pair domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 166438 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-6P-1
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Backbone manufacturerAmersham-Pharmacia
- Backbone size w/o insert (bp) 4984
- Total vector size (bp) 5431
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameToxoplasma gondii CBS-pair domain
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SpeciesToxoplasma gondii
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Insert Size (bp)447
- Promoter Tac, LacI, Amp
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX Fwd
- 3′ sequencing primer pGEX Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6P-1 Toxoplasma gondii CBS-pair domain was a gift from Kalle Gehring (Addgene plasmid # 166438 ; http://n2t.net/addgene:166438 ; RRID:Addgene_166438) -
For your References section:
Burst kinetics and CNNM binding are evolutionarily conserved properties of phosphatases of regenerating liver. Fakih R, Goldstein RH, Kozlov G, Gehring K. J Biol Chem. 2023 Apr;299(4):103055. doi: 10.1016/j.jbc.2023.103055. Epub 2023 Feb 22. 10.1016/j.jbc.2023.103055 PubMed 36822330