-
Plasmid#174024PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserteGFP
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csChBP
Plasmid#108876PurposeExpression of APEX2 fused to a conditionally stable nanobody against mCherryDepositorInsertAPEX2-csChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp Phy-mCherry-CAAX
Plasmid#50839PurposeOptogenetic PI3K activation system; membrane componentDepositorInsertPhyB 1-908
UseLentiviral and Synthetic BiologyTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) EC Her2-Fc
Plasmid#183250PurposeSoluble expression of the extracellular domain of the HER2 antigen. Human IgG1 Fc.DepositorInsertHer2 antigen Fc
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-SENP3
Plasmid#115521PurposeMammalian expression constructDepositorInsertSENP3 (Senp3 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR iSH-YFP-PIF
Plasmid#50841PurposeOptogenetic PI3K activation system; cytoplasmic componentDepositorInsertsp85 iSH2
PIF6 1-100
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
ecAT3.10
Plasmid#107215PurposeMammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.DepositorInsertecAT3.10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
csr-1_hH1_mEos3.1_V5
Plasmid#191750PurposePhotoconvertible fluorescent nuclear reporter.DepositorInserthH1 (hH1 Neurospora crassa)
UseTagsV5, ccg-1 promoter, and mEos3.1ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mNeonGreen
Plasmid#191743PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mNeonGreenExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_human
Plasmid#71509PurposehumanSTARR-seq screening vector with SCP1 core promoterDepositorTypeEmpty backboneUseHumanstarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
EcpH-TM
Plasmid#85389PurposeExpresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 1) in mammalian cells. Incorporates into viral membrane to label pseudovirusesDepositorInsertecliptic pHluorin (EcpH) fused to N-terminus of human ICAM-1 transmembrane domain
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mTagBFP2
Plasmid#191746PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mTagBFP2ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_vsfGFP9
Plasmid#191747PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and vsfGFP-9ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmCherry1
Plasmid#89986PurposeLong stokes-shift variant of red fluorescent protein mCherryDepositorInsertLSSmCherry1
UseTags6xHis-T7-XpressExpressionBacterialMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO SwiChRca-TS-EYFP
Plasmid#55631PurposeCre-Dependent Chloride ChannelDepositorInsertiC1C2-TS-EYFP
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationC128APromoterEf1aAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
469_pME_iCre
Plasmid#171792PurposepME-Vector with codon improved iCre sequence. Tol2-kit compatible.DepositorInsertcodon-optimized iCre sequence
UseTagsExpressionMutationPromoterNoneAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRU44
Plasmid#167666PurposeENTRY vector for gRNA4 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-TF
Plasmid#83921PurposeCo-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.DepositorInsertsSkp
Trigger Factor
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRU43
Plasmid#167665PurposeENTRY vector for gRNA3 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only