-
Plasmid#13404DepositorInsertUpstream region of mouse Fbx15 gene
UseLuciferaseTagsExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-H2B-MCS
Plasmid#184041PurposeCloning of GFP-H2B fusion proteinsDepositorTypeEmpty backboneUseTagsGFP and H2BExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC10
Plasmid#197404PurposeHoney bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and pVS1 repliconDepositorInsertpVS1 replicon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
OA-1067B
Plasmid#200252PurposepBac-U6-gRNA(doublesex)-3xp3-tdTomatoDepositorInsert4 gRNAs targeting Doublesex
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtTIM21(1-50aa)-mCherry
Plasmid#194047PurposeTransient expression of AtTIM21-mCherry in plant cell (Mitochondria)DepositorInsertmCherry
UseTagsAtTIM21 1–50aaExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_DUX4_KBM
Plasmid#187800PurposeDUX4 with KBM (p. E414-L424 deletion) with MAC-C tagDepositorInsertDUX4-KBM (DUX4 Human)
UseTagsExpressionMammalianMutationp. E414-L424 deletionPromoterAvailable sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_NTRK3
Plasmid#187796PurposeMAC-tagged gene expression of human NTRK3DepositorInsertNTRK3 (NTRK3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pSB3K3-CT81
Plasmid#165404PurposeBacteria gene circuit on medium copy backbone.DepositorInsertCT81 gene circuit
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
PHY55-mouse U6-sgLMNA
Plasmid#164045PurposeU6-driven sgRNA targeting LMNADepositorInsertLMNA targeting sgRNA
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-W340A.pcDNA3-k
Plasmid#127404PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.DepositorInsertMaltose Binding Protein
UseTagsFLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon and W34…PromoterCMVAvailable sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAEL017313 GPCR
Plasmid#115404PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertAAEL017313 GPCR (LOC23687733 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFusX3-59
Plasmid#63404PurposeEncodes RVD positions 7-9 in TALEN assemblyDepositorInsertNG NN NN
UseTALENTagsExpressionMutationnonePromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Crkl SH2SH3
Plasmid#36404DepositorInsertCrkl SH2 and N-terminal SH3 domains (CRKL Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
enz.043-SETD7-Y305F
Plasmid#154045PurposeExpresses SETD7 (Y305F) in E. coliDepositorInsertHistone-lysine N-methyltransferase SETD7 (SETD7 Human)
UseTagsHis, MBPExpressionBacterialMutationchanged tyrosine 305 to phenylalaninePromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
UseTags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2PromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEE035
Plasmid#176792PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert404
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLuc-Optop38i-dsm
Plasmid#89748PurposeExpression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13FDepositorInsertOptop38i dark-state mutant
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-36: AAVS1-mEGFP (PGK)
Plasmid#114404PurposeHomology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -