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gRNA Cloning Vector Bbs I ver. 2

Plasmid

#85586

Purpose

An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pJWV102-PL-dCas9

Plasmid

#85588

Purpose

Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

dCas9sp

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PLsp

Availability

Academic Institutions and Nonprofits only

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pPEPX-P3-sgRNAluc

Plasmid

#85590

Purpose

Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

sgRNA targeting firefly luciferase encoding gene

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

P3

Availability

Academic Institutions and Nonprofits only

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pEJS427-pCSDest2-AcrE2

Plasmid

#85677

Purpose

Mammalian expression of anti-CRISPR protein from Type I-E

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type I-E anti-CRISPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEJS430-pCSDest2-AcrIIC1Boe

Plasmid

#85678

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC1Boe

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEJS433-pCSDest2-AcrIIC1Nme

Plasmid

#85679

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC1Nme

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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sgOpti

Plasmid

#85681

Purpose

Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

Depositor

David Sabatini, Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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pEJS436-pCSDest2-AcrIIC2Nme

Plasmid

#85712

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC2Nme

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS443-pCSDest2-AcrIIC3Nme

Plasmid

#85713

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC3Nme

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

Plasmid

#85714

Purpose

Telomere-targeting Nme sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

Plasmid

#85715

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one

Plasmid

#85716

Purpose

Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Nme dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO and U6

Availability

Academic Institutions and Nonprofits only

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pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

Plasmid

#85717

Purpose

Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Sp dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Mutation

Promoter

CMV-TO and U6

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(WT)-EGFP

Plasmid

#89633

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-dCas9-MQ1(C141S)-EGFP

Plasmid

#89635

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-dCas9-MQ1(S317A)-EGFP

Plasmid

#89636

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Mutation

S317A, AGC-->GCC

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-dCas9-dMQ1-EGFP

Plasmid

#89637

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC; S317A, AGC-->GCC

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
p426-SNR52p-gRNA.clr-2.Y-SUP4t

Plasmid

#89692

Purpose

gRNA for clr-2 locus

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

gRNA for clr-2 locus

Use

CRISPR; Grna

Tags

No

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pX335A_hCas9(D10A)_IRF8gRNA1

Plasmid

#89719

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Article

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA1 (IRF8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pX335A_hCas9(D10A)_IRF8gRNA2

Plasmid

#89720

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Article

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA2 (IRF8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge