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Showing: 10941 - 10960 of 34765 results

  1. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

    Plasmid
    #182346
    Purpose
    PacI-flanked CAG-LSL expression cassette with a FNF for positive selection
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182346-plasmid-map-sequence-id-358221

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  3. SDCBPA-c021

    Plasmid
    #175485
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), G111-F275.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175485-plasmid-map-sequence-id-353207

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  4. SDCBPA-c011

    Plasmid
    #175483
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), A106-F275.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175483-plasmid-map-sequence-id-353170

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  5. pCDX4-donor

    Plasmid
    #176343
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    CDX4 (CDX4 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176343-plasmid-map-sequence-id-347886

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  6. pJAZF1-donor

    Plasmid
    #176352
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    JAZF1 (JAZF1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176352-plasmid-map-sequence-id-347876

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  7. pB2HoptX

    Plasmid
    #163832
    Purpose
    Inactive two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    MidT Substrate Y4F
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163832-plasmid-map-sequence-id-337598

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  8. pGBK CUP1 mCherry

    Plasmid
    #170264
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mCherry
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Insert
    momeric Cherry
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170264-plasmid-map-sequence-id-335100

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  9. pHS29

    Plasmid
    #171205
    Purpose
    bacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motif
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    meg-3 (meg-3 Nematode)
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    F705A,Y708A,Y713A, N725A
    Promoter
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171205-plasmid-map-sequence-id-335784

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  10. HCP4

    Plasmid
    #166106
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166106-plasmid-map-sequence-id-330381

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  11. HCP6

    Plasmid
    #166108
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166108-plasmid-map-sequence-id-330318

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  12. C-BRPF3-8

    Plasmid
    #166516
    Purpose
    scFv of a human scaffold targeting Bromodomain and PHD finger-containing protein 3. Antigen coverage aa 1056-1195 of 1205, PWWP domain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166516-plasmid-map-sequence-id-328228

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  13. pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)

    Plasmid
    #160631
    Purpose
    Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.
    Depositor
    Diego Orzaez
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U626
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160631-plasmid-map-sequence-id-321967

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  14. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid
    #160640
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160640-plasmid-map-sequence-id-321670

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  15. 6WAT

    Plasmid
    #162268
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    PHF1 (PHF1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    162268-plasmid-map-sequence-id-319831

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  16. 6PED

    Plasmid
    #162265
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    HEMK2 (N6AMT1 Human)
    Use
    Tags
    6xHis-Thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    162265-plasmid-map-sequence-id-319828

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Showing: 10941 - 10960 of 34765 results