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r. pT7-7-SNCA-5Asn

Plasmid

#107295

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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s2. pT7-7-SNCA-5Asp

Plasmid

#107293

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asp (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgFTCD_1

Plasmid

#102312

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_1 (FTCD Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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FB012

Plasmid

#119709

Purpose

Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PtrpC:hph:Ttub

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf178

Plasmid

#12866

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf178

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV mCherry R4-R3

Plasmid

#62147

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

mCherry

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 LacZ L5-L2

Plasmid

#62167

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and LacZ module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

LacZ

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBS SDF1

Plasmid

#19202

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

SDF1 (CXCL12 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-Barkor 1-91

Plasmid

#24395

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Contains amino acids 1-91.

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Barkor 1-180

Plasmid

#24396

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Contains aa 1-180.

Promoter

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pM-ErbB4-delta-kinase

Plasmid

#17800

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pPK1E-1+t-gfp

Plasmid

#48131

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 TEV site pET15b

Plasmid

#60685

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26I DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26I and TEV site was inserted (see comments belo…

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pNAS1b split mCherry NEDD4 WW2

Plasmid

#111882

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Use

Tags

Split mCherry system: NEDD4 WW2 fused to C-termin…

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 delta C22

Plasmid

#19942

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 delta C22 (CUL1 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

Mutation of 22 amino acids from the C terminus

Promoter

Available since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR SV40 tdTomato L5-L2

Plasmid

#62154

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and tdTomato module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223 PAK5 T538N

Plasmid

#60537

Purpose

Donor vector for human Pak5 T538N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

T538N

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S573N

Plasmid

#60538

Purpose

Donor vector for human Pak5 S573N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S573N

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pPKm-235

Plasmid

#90509

Purpose

pSIN - EF-1alpha - MTS sHO1 - P2A - MTS - Hy2, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and phytochromobilin:ferredoxin oxidoreductase (Hy2)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-sHO1, mito-Hy2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-hTIG3-1-164

Plasmid

#100724

Purpose

Full-length human TIG3 in E.coli expression vector

Depositor

Byung Il Lee

Article

Han et al Protein Expr Purif. 2010 May;71(1):103-7

Insert

RARRES3 (PLAAT4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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