-
Plasmid#72019PurposeExpresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3e (Sema3e Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE32-pr-mEosFP (mEosFP-V69T)
Plasmid#99213PurposeBacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).DepositorInsertpr-mEosFP
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T for primed c…PromoterT5Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-AD8-QES.i08.c04
Plasmid#123259PurposeMammalian expression plasmid for Env from the AD8 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertHIV-1 (AD8) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Mutations P124D, …PromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS2
Plasmid#127127DepositorInsertRPS2 (RPS2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP*-PTEN
Plasmid#86781PurposeFusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.DepositorInsertPTEN (PTEN Human)
UseTagsFKBP* (F36V and L106P) and linkerExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2616
Plasmid#91090PurposeModule C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertBsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterAt7SLAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p8325 pLIX YAP1 WT
Plasmid#184527PurposeExpression of YAP1 WTDepositorInsertYAP1 WT (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE promoter, Tet ONAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTET2-RlucCP
Plasmid#198352PurposePart of Firefly reporter system, encodes Renilla luciferaseDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe human FOXC2
Plasmid#15535DepositorInsertFOXC2 (FOXC2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 S135A
Plasmid#155317PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationS135APromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorInsertG4G6 (GSN Human)
UseTags6xHis-MBPExpressionBacterialMutationWTPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
ETV1_pet28a
Plasmid#131644PurposeBacterial expression of ETS gene ETV1DepositorInsertETV1 (ETV1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ETV6-ETS
Plasmid#111180PurposeMinimal ETS domain of human ETV6 (TEL)DepositorInsertETV6 ETS domain (ETV6 Human, Mouse, Bovine)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNH11 (pmyo-2::Arch(D95N)::2xMycTag)
Plasmid#130275PurposeExpresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.DepositorInsertArch(D95N)
UseTags2x Myc-tagExpressionWormMutationPromoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD2529-CAG-aIIb
Plasmid#189796PurposeExpression of full length human integrin aIIbDepositorInsertaIIb (ITGA2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Adeno EA
Plasmid#64071PurposeAdenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4DepositorInsertU6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
UseAdenoviralTagsFLAG-Cas9ExpressionMutationPromoterU6 and CBhAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA
Plasmid#47457PurposeLITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.DepositorInsertNLS(alpha-imp)-CRY2PHR-NLS-VP64
UseTags2A_GFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38-AtLEA4-5
Plasmid#178189PurposeSED1 biosensor for expression in Saccharomyces cerevisiaeDepositorInsertSED1 biosensor
UseTagsExpressionYeastMutationPromoterpPMA1Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
GST-BHMT-I/G
Plasmid#104442PurposeAutophagy cargo assayDepositorInsertBHMT (BHMT Human)
UseTagsGFP-myc GFP is from Aequorea victoria and GSTExpressionMammalianMutationN43A in GST (in a potential KFERQ-like sequence).…PromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only