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pLPC-N-Myc-Flag-BirA-hPOT1

Plasmid

#166409

Purpose

express BirA-hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Tags

BirA, flag, and myc

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf76

Plasmid

#12764

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf76

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
XE223 human myc-Chibby-pCS2+MT

Plasmid

#16825

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

myc-Chibby (CBY1 Human)

Use

Xenopus expression

Tags

6-myc

Expression

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pBS ColI alpha2

Plasmid

#19207

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 type I collagen (COL1A2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pSC50

Plasmid

#104824

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.08g081600, Glyma.05g126600

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEFU6-EGFP_shPKA-Cα

Plasmid

#114142

Purpose

Expression of shRNA for knocking down endogenous PKA-Cα. EGFP is also expressed.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

shRNA expression for knock down mouse PKA-Cα

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV CreERT2 L5-L2

Plasmid

#62172

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

CreERT2

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf98

Plasmid

#12786

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf98

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

Plasmid

#82469

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mRNA capping enzyme (Rngtt Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

changed Lysine 294 to Alanine

Promoter

CMV

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc NES-mCE NLS-Flag

Plasmid

#82468

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-NES-mCE-Flag (Rngtt HIV, Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRAN357

Plasmid

#120898

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein

Depositor

David S. Weiss, Craig Ellermeier

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAc-HT-Dmp53KRKR

Plasmid

#85699

Purpose

expression of Drosophila p53 KRKR mutant in Drosophila cells

Depositor

Giannino Del Sal, Licio Collavin

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

Dmp53 (p53 Fly)

Use

Tags

His

Expression

Insect

Mutation

K26R K302R

Promoter

Ac5

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV eGFP L5-L2

Plasmid

#62140

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV PuroR L3-L2

Plasmid

#62163

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and Puromycin module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Puromycin

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf78

Plasmid

#12766

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf78

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pPN460

Plasmid

#137878

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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Stat92e pPAcPL

Plasmid

#8713

Depositor

Jim Darnell

Article

Yan et al Cell 1996 Feb 9;84(3):421-30.

Insert

Stat92e (Stat92E Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 20, 2005

Availability

Academic Institutions and Nonprofits only
pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

Plasmid

#166412

Purpose

express hPOT1 K90E in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 K90E (POT1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

K90E

Promoter

EF-1a

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPK1E-1-gfp

Plasmid

#48133

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pBS ALS2

Plasmid

#19228

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Amyotrophic lateral sclerosis 2 (juvenile) (ALS2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only