-
Plasmid#37307DepositorInsertGAI (GAI Mustard Weed)
UseTagsCFPExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
SAC1C389SdeltaTMD-FKBP-mCherry
Plasmid#108124PurposeRecruitable cytosolic inactive SAC1DepositorInsertSACM1L(C389S; 1-520):FKBP1A(3-108):mCherry (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(C389S; 1-520)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDOC-GG
Plasmid#149377PurposeChassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene DoctoringDepositorInsertsFragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJG363
Plasmid#91183PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)DepositorInsertnAtCas9_D10A+gNt_R2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG370
Plasmid#91187PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)DepositorInsertnAtCas9_D10A+gRNActrl+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5
Plasmid#135455PurposeBacterial expression of uTEV1Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, S153N mutation…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-syn-fRCaMP2
Plasmid#125246PurposeExpresses red fluorescent calcium sensor in hippocampal slicesDepositorInsertfRCaMP2
UseTagsExpressionMammalianMutationD396APromotersynapsinAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-E6AP Hect
Plasmid#165102Purposebacterial expression of a GST fusion of Human E6AP Hect domainDepositorInsertE6AP Hect (UBE3A Human)
UseTagsGST from plasmid.ExpressionBacterialMutationE6AP Hect domain Amino acids 495-738 (5'QLNP…PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Perox-B
Plasmid#209867PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3DepositorInsertddGFP B
UseTagsTargeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP5H
Plasmid#188061PurposePROSS-designed versatile peroxidase 5HDepositorInsertPROSS-designed versatile peroxidase from Pleurotus ostreatus
UseTagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation43 PROSS mutations, described in publicationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-AKAR4
Plasmid#64733PurposeEndoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.DepositorInsertAKAR4
UseTagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
RS18960_pMQ30_KO_Kan
Plasmid#185393PurposeNon-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11DepositorInsertsRR42_RS18960 upstream flanking homology region
RR42_RS18960 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYDE007
Plasmid#194152PurposesgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.DepositorInsertsgRNA with mrfp control guide sequence
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1_IS_RT
Plasmid#97091PurposeEncodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.DepositorInsertNEAT1_IS_RT (NEAT1 Human)
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP11H
Plasmid#188063PurposePROSS-designed versatile peroxidase 11HDepositorInsertPROSS-designed versatile peroxidase from Pleurotus ostreatus
UseTagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPb-FKBP-post mGRASP
Plasmid#73146PurposeSmall fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.DepositorInsertsHRPb-FKBP-post mGRASP
UseTagsHA tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRES-Ap2a2-FKBP
Plasmid#46944PurposeCo-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cellsDepositorInsertAp2a2-FKBP
UseTagsMainly mouse Ap2a2 cDNA, with a fragment of brain…ExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1
Plasmid#183400PurposeDual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-lambdaRED_NOC
Plasmid#127032PurposeBasic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).DepositorInsertPart ?RED_NOC
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only