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PGK Promoter (MTK 2) (pAN2822)

Plasmid

#194224

Purpose

Mammalian Toolkit part 2 encoding the PGK promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PGK

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a Promoter (MTK 2) (pAN2824)

Plasmid

#194225

Purpose

Mammalian Toolkit part 2 containing the EF1a promoter

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LCB1_v1.3 (MTK 3a) (pZYW044)

Plasmid

#194227

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

LCB1_v1.3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LCB3_v1.2 (MTK 3a) (pZYW045)

Plasmid

#194228

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

LCB3_v1.2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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WPRE (MTK 4b) (pAN2410)

Plasmid

#194235

Purpose

Mammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbones

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

WPRE terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-vp16

Plasmid

#171075

Purpose

Expresses Gal4-vp16 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Use

Tags

His and VP16 AD (413-490)

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-p53

Plasmid

#171076

Purpose

Expresses Gal4-p53 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Use

Tags

His and p53(1-85)

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_zCas9

Plasmid

#213913

Purpose

Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system

Depositor

Sébastien Mongrand

Article

Ziqiang P. et al Bio Protocol, Vol 14, Iss 13, Jul 5, 202

Insert

zCas9

Use

CRISPR

Tags

Expression

Mutation

Zea mays codon-optimized Cas9

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL452

Plasmid

#219502

Purpose

Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-TtrpC

Plasmid

#213467

Purpose

Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Terminator of tryptophan synthase of Aspergillus nidulans

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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