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Plasmid#188755PurposeLentiviral backbone containing NOTC2 Notch domain as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertNOTC2 Notch (NOTCH2 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6f
Plasmid#207856PurposeMammalian expression of PE6f prime editorDepositorInsertPE6f
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6g
Plasmid#207857PurposeMammalian expression of PE6g prime editorDepositorInsertPE6g
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-AR
Plasmid#28235PurposeMammalian expression of Androgen receptor fused to EGFPDepositorInsertAndrogen receptor (AR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
UseTags14xHis-Avi-SUMOEuExpressionBacterialMutationPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag Depdc5 pRK5
Plasmid#46340DepositorInsertDepdc5 (DEPDC5 Human)
UseTagsFlagExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
LAMP-mChF-AIP
Plasmid#61524PurposeExpression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIPDepositorInsertlysosomal-associated membrane protein 1 (LAMP1 Human)
UseTagsAIP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (396-492)
Plasmid#86755PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 396-492PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (181-413)
Plasmid#86754PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 181-413PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (201-492)
Plasmid#86751PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 201-492PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMP71-HLA-A0201-His
Plasmid#108214PurposeHLA-A0201 his taggedDepositorInsertmajor histocompatibility complex, class I, B (HLA-A Human)
UseRetroviralTagshisExpressionMammalianMutationPromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF
Plasmid#74924PurposeMammalian expression of human NSF full-length (744 aa)DepositorInsertNSF (NSF Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a]
Plasmid#49332PurposeRepaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…PromoterCMVAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1fl
Plasmid#109157PurposeEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIA10.7
Plasmid#214451PurposeBxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.DepositorInsertsTet-On 3G
PuroR
TRE3G CopGFP
UseBxbi donorTagsPuroR, T2A, and Tet-On 3GExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
UseTagsH6 and ybbRExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep128
Plasmid#136872PurposeMammalian expression of the centrosomal protein Cep128 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep128 (CEP128 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
Plasmid#221081PurposeSEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1
Plasmid#184395PurposeDonor plasmid for stable integration of firefly luciferase control reporter at AAVS1DepositorInsertFirefly luciferase beta-globin
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-DAXX
Plasmid#169726PurposeMammalian expression construct of GFP tagged full length DAXXDepositorInsertDAXX (DAXX Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only