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Plasmid#205724PurposeExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylaseDepositorInsertpOTC-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xABRE_R
Plasmid#185804Purposea synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the RD29A promoterDepositorInsert6xABRE(RD29A)+-86MP+erGFP
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(80-421)
Plasmid#209005PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep
Plasmid#208644Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
UseCRISPR; Ssdna donnor preparationTagsTwin StrepExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-msEGFP1.8
Plasmid#208619PurposeMammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.DepositorInsertmsEGFP1.8
UseTagsExpressionMammalianMutation(compared with pEGFP-C1, protein level) S30R, Y39…PromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-EGFP-Cre
Plasmid#207132PurposeLentiviral vector to express EGFP-CreDepositorInsertEGFP-Cre
UseLentiviralTagsExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZa pLtetO CsgA-SpyTag
Plasmid#204503PurposeBacterial expression of CsgA with C-terminal SpyTag peptide and GS linkerDepositorInsertCsgA
UseSynthetic BiologyTags6xHis, SpyTagExpressionBacterialMutationPromoterpLtetOAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPO41 deltaN380
Plasmid#205076PurposeRPO41 protein expressionDepositorInsertRPO41 (RPO41 Budding Yeast)
UseTagsExpressionBacterialMutationLacks N Terminal 380 amino acidsPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPO41 deltaN296
Plasmid#205075PurposeRPO41 protein expressionDepositorInsertRPO41 (RPO41 Budding Yeast)
UseTagsExpressionBacterialMutationLacks N Terminal 296 amino acidsPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(80-421)msGFP
Plasmid#209009PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis and msfGFPExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(96-421)
Plasmid#209006PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 96-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80-142)
Plasmid#209012PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-msfGFPExpressionBacterialMutationaa 80-142 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(143-421)
Plasmid#209007PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
G1095C_BattB(GA)_jGCaMP7c_IRES_shBleR-P2A-HygroR
Plasmid#200638PurposejGCaMP7c recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cellsDepositorInsertjGCaMP7c
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tc-INTR-GFP
Plasmid#208899Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the beetle Tribolium castaneum, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
CSII-TK-MCS
Plasmid#203597PurposeEmpty lentiviral vector to express protein in mammalian cells cells.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterTKAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA010.188
Plasmid#206792PurposeJ23113.dCas9DepositorInsertdCas9
UseTagsExpressionMutationPromoterJ23113Available sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK389
Plasmid#206791PurposeSp.dCas9, pTet.MCP-SoxS, J23119.gRNADepositorInsertsdCas9
MCP-SoxS
scRNA
UseTagsExpressionMutationPromoterJ23119 and TetAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT009.119.DA9
Plasmid#206803PurposeExpresses DA9 scRNA for activation of pDA303DepositorInsertDA9 scRNA
UseTagsExpressionMutationPromoterJ23119Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA303.DA9.1B.mRFP
Plasmid#206802PurposemRFP with synthetic promoter HP3 (from Figure S21)DepositorInsertmRFP
UseTagsExpressionMutationPromoterDA9.1B (HP3)Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only