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Plasmid#159821PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertMCS mosTI unc-119 target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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V5-LOV-Turbo-NES
Plasmid#199545Purposeexpresses LOV-Turbo in the mammalian cytosol, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsNES and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
Plasmid#194519PurposeDual fluorescence reporter for studying protein degradationDepositorInsertTIF4631 leader sequence
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
15x-QUAS-dTomato-T2A-TRPV1
Plasmid#140945PurposeQUAS reporter line that expresses cytosolic dTomato and the rat TRPV1 ion channel, separated by T2ADepositorInsert15x-QUAS-dTomato-T2A-TRPV1
UseTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo1-G3BP1_pTRE-G418
Plasmid#202015Purposeexpresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertV5-LOV-Turbo1-G3BP1
UseLentiviralTagsV5ExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES-Ap1g1-FKBP
Plasmid#46945PurposeCo-expresses siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cellsDepositorInsertAp1g1-FKBP
UseTagsMainly mouse Ap1g1 cDNA, with a fragment of brain…ExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTLR repair vector
Plasmid#64322PurposeVector for repair of the traffic light reporter (TLR), providing the venus coding regionDepositorInsertVenus
UseArtificial targeting vectorTagsExpressionMutationsee publication for detailsPromoternoneAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-ERM
Plasmid#199664Purposeexpresses LOV-Turbo on the mammalian ER membrane, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsSEC61B and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-NLS
Plasmid#199663Purposeexpresses LOV-Turbo in the mammalian nucleus, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsNLS and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mito-CaNAR2
Plasmid#64731PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNAR2
UseTagsCerulean3, N-terminal 30 amino acids from DAKAP1,…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDL17
Plasmid#106483PurposepDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosomeDepositorInsertsgam
bet
exo
lacI
rhaR
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-a (p15A origin)
Plasmid#74088PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_mTurquoise2
Plasmid#113895PurposeMammalian (inducible) expression of cytosolic mTurquoise2DepositorInsertmonomeric Turquoise2
UseLentiviralTagsExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
Plasmid#159807PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_EmGFP
Plasmid#113892PurposeMammalian (inducible) expression of cytosolic EmGFPDepositorInsertEmerald GFP
UseLentiviralTagsExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1
Plasmid#183402PurposeDual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b-Casp8 delta DED
Plasmid#11827DepositorInsertCaspase 8 minus Death Effecter Domain (CASP8 Human)
UseTags6xHisExpressionBacterialMutationMinus Death Defecter Domain (aa 1-216)PromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUDP004
Plasmid#101165PurposeE. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp7-His
Plasmid#11825DepositorInsertCaspase 7 (CASP7 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only