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RIPK4 gRNA (BRDN0001147121)

Plasmid

#75969

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK4 (RIPK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-ATP5O

Plasmid

#139842

Purpose

Lentiviral expression of human ATP5O

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

ATP5O (ATP5PO Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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WPXL LEFTY2 gRNA

Plasmid

#101924

Purpose

LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

LEFTY2 enhancer targeting gRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001149005)

Plasmid

#80180

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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DYRK3 gRNA (BRDN0001145918)

Plasmid

#75547

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK3 (DYRK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pUCHR-mClover-smAID-P2A-CD5HA2-bglpA

Plasmid

#110655

Purpose

Lentiviral vector expressing cell surface HA-tag and monomeric GFP C-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

mClover-AID-P2A-CD5HA2-bglpA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLgw ELK4-V5-EcoDam

Plasmid

#98607

Purpose

Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Lentiviral; Damid

Tags

V5 and Dam (DNA adenine methyltransferase)

Expression

Mammalian

Mutation

Promoter

Heat Shock Minimal Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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LCV2_AAVS1_sgRNA_2

Plasmid

#155088

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-KS

Plasmid

#136617

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4-IRES-Sox2 (Klf4, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OKM

Plasmid

#136554

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-KSM

Plasmid

#136553

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4-IRES-Sox2-E2A-cMyc (Klf4, Sox2, Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OSM

Plasmid

#136555

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-E2A-cMyc (Myc, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex5

Plasmid

#85535

Purpose

Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTPN11-T73I

Plasmid

#116647

Purpose

Lentiviral expression of PTPN11 T73I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTPN11 (PTPN11 Human)

Use

Lentiviral

Tags

Expression

Mutation

T73I

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-GFP-shDyn1

Plasmid

#132711

Purpose

Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Pdyn gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shDyn(1) (Pdyn Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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MAP3K5 gRNA (BRDN0001148435)

Plasmid

#76654

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K5

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K5 (MAP3K5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX SC4MOL 519

Plasmid

#27984

Depositor

Igor Astsaturov

Insert

SC4MOL (MSMO1 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Mutation

H173Q mutation in SC4MOL

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pLN481 (FuGW-G8p-IL12-1Pe)

Plasmid

#105191

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-IL12-1Pe

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfRab8b_3

Plasmid

#26709

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rab8b RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Dog Rab8b_3 RNAi.

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
TRIM33 gRNA (BRDN0001162242)

Plasmid

#77099

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM33

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM33 (TRIM33 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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