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Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRVL-2
Plasmid#104580PurposeContains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorInsertMouse IgG2c CH1/CH2/CH3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pETx-SR6v15
Plasmid#176212PurposeBacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6v15
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Firefly_luc-60A
Plasmid#200395PurposeA plasmid IVT template containing a Firefly luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE GS-gRNA (hSyn-Cre) backbone
Plasmid#200368PurposeBackbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPCK
Plasmid#133128Purposea recombinant plasmid, expressing pckA PEP-carboxykinase) geneDepositorInsertPEP-carboxykinase gene
UseTagsExpressionBacterialMutationPromoterPEP-carboxykinase promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF0
Plasmid#200371PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Adeno Cas9
Plasmid#64072PurposeAdenoviral vector for the expression of the Cas9DepositorTypeEmpty backboneUseAdenoviralTagsFLAGExpressionMutationPromoterU6 and CBhAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT005-HaloGPM
Plasmid#163385Purpose2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN systemDepositorInsertHalotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 WAVE 2
Plasmid#67098PurposeStandard for cell-free expression benchmarking.DepositorInsertWAVE 2 (WASF2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA::CYP71AV1/CPR
Plasmid#20118DepositorInsertamorphadiene oxidase and p450 reductase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PE2-C
Plasmid#164908PurposeExpresses the C-terminal split-intein fragment of PE2DepositorInsertPE2-C
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-CasRX-P2A-EGFP-BGH PolyA
Plasmid#154004PurposeExpress CasRxDepositorInsertCasRx
UseCRISPRTagsExpressionMutationPromoterCAGAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF2
Plasmid#200373PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0103
Plasmid#91000PurposeModule A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM552
Plasmid#154131PurposeExpresses wt rRNAs operon in bacterial cellsDepositorInsertwt bacterial rRNAs operon under control of pL promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-flex-oChIEF-citrine
Plasmid#50973Purposeflexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITRDepositorInsertChIEF-citrine
UseAAVTagscitrineExpressionMutationinverted and inserted between two lox sitesPromoterhuman synapsin promoterAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BaLgp160
Plasmid#100919PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolateDepositorInsertHIV-1 (BaL) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only