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Plasmid#162992PurposeBEAR control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-RRBP1-1
Plasmid#92156PurposeCRISPR guide RNA targeting human RRBP1DepositorInsertRRBP1 sgRNA-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208 CMV sTurboID (C)-HA-FRB-ERM
Plasmid#153007PurposeExpresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membraneDepositorInsertsTurboID (C) (G74)-ERM (Cb5)
UseLentiviralTagsFRB and HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV OMM-FKBP-V5-sTurboID (N)
Plasmid#153006PurposeExpresses split-TurboID N-terminal fragment targeted to outer mitochondrial membraneDepositorInsertOMM (Tom20)-sTurboID (N) (L73)
UseLentiviralTagsFKBP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDAS12489_PEAR-GFP_2in1_2.0
Plasmid#177186PurposePEAR-GFP3 plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)
Plasmid#153005PurposeExpresses high-affinity split-TurboID C-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (C) (G79)
UseLentiviralTagsFRB, HA, and HaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
hHS1
Plasmid#159169PurposeHuman codon-optimized cytosolic labile heme reporterDepositorInserthHS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-1b-RFC140ΔN555
Plasmid#175043PurposeOverexpression of human (Rfc1) p140ΔN555 in E.coliDepositorInsertReplication factor C subunit 1 (RFC1 Human)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS:mCherry
Plasmid#170994PurposeUAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.DepositorInsertUAS:mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterSynthetic 14x UAS:Gal4Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFAB808
Plasmid#47853PurposeBIOFAB reporter plasmid for measuring rrnB T1 termination efficiencyDepositorInsertrrnB T1 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO8Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSEM319 - array co-injection fragment mosTI unc-119
Plasmid#159823PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertarray co-injection fragment mosTI unc-119
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hSyn-NCreIntN-T2A-mTagBFP2
Plasmid#160502PurposeExpresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterhSynAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
UseTagsHA tag and myc tagExpressionYeastMutationPromoterGAL1Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
UseTagsExpressionBacterialMutationA309GPromoterAvailable sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1
Plasmid#158738PurposeMammalian expression of scFLARE1 componentDepositorInsertscFLARE1
UseAAVTagsFlag, V5ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5
Plasmid#135456PurposeBacterial expression of uTEV2Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, T30A/S153N mut…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM272 - MCS mosTI Pmlc-2_GFP target
Plasmid#159834PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertMCS mosTI Pmlc-2_GFP target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTMEM-PhoCl-Insert
Plasmid#219674PurposeCaging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarletDepositorInsertTMEM-PhoCl-rtTA3
UseTagsPhoClExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits