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Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
UseTagsExpressionBacterialMutationPromoterGlnS (constitutive)Available sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
UseTags14Histidine-TEVExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-cMyc
Plasmid#46970Purposelentiviral expression of c-MycDepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT WT
Plasmid#84924Purposeluciferase reporter for TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6g
Plasmid#207857PurposeMammalian expression of PE6g prime editorDepositorInsertPE6g
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6f
Plasmid#207856PurposeMammalian expression of PE6f prime editorDepositorInsertPE6f
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-GFP-IRF3
Plasmid#127663PurposeOver-express EGFP-IRF3DepositorInsertIRF3 (IRF3 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-BSD (BRD4)
Plasmid#91795PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-BSD
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro
Plasmid#228401PurposeEncodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1DepositorInsertSREBP1 (SREBF1 Human)
UseTagsmNeonGreen2xExpressionMammalianMutationdeleted N terminal activation domain (amino acids…PromoterEF1aAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry
Plasmid#122132PurposeExpress a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoterDepositorInsertdCas9-10X GCN4-P2A-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationdCas9 D10A & H840APromoterTRE3GAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFP/Cre
Plasmid#49056Purposeadeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.DepositorInsertCre
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP, Myc, and NLSExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC35F2
Plasmid#132121PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC35F2 (SLC35F2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRM210 LGP (Luciferase GFP Puro)
Plasmid#174722PurposeExpresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance geneDepositorInsertsFirefly Luciferase
eGFP
pac
UseLentiviral and LuciferaseTagsE2A linker and F2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e
Plasmid#181751PurposeMMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-lamin A
Plasmid#175107PurposeLentiviral expression of V5-tagged mouse LmnaDepositorInsertLmna (Lmna Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-GFP-P2A-bReaches-minWPRE
Plasmid#118276PurposeExpresses EGFP and bReaChES under a minimal CamKII PromoterDepositorInsertsEGFP
bReaCHES
UseAAVTagsP2AExpressionMutationPromoterCamK IIAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e-NG
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV9retro
Plasmid#224687PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap9retro
UseAAVTagsExpressionMutationinsertion of 10-mer peptide from AAV2retro (LADQD…PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNMSB84
Plasmid#199309PurposeMosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertflp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…Promoterflp-18Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-pHSE::Cre-mRuby3
Plasmid#164136PurposeLentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat inductionDepositorInsertHSE promoter-Cre-T2A-mRuby3
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterHSE synthetic promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only