Showing: 1061 - 1080 of 1302 results
-
Plasmid#108408PurposeCan be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.DepositorInsertPKHD1 (PKHD1 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSF4 CMV 5TOP intron renilla TRICK CTE polyA
Plasmid#64544Purpose5′ TOP TRICK reporter mRNADepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, 5' TOP (5′ terminal oligo…ExpressionMammalianMutationPromoterTetracycline-inducible promoter and 5′ UTR of hum…AvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_AB
Plasmid#66008PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_EB
Plasmid#66009PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_FB
Plasmid#66010PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_GB
Plasmid#66011PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C0040 (tetR)_CD
Plasmid#66027PurposeMoClo Basic Part: CDS - Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) [C:C0040:D]DepositorInsertTranscription factor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-NIrD
Plasmid#65010PurposeNS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infectionDepositorInsertsrtTA-MAVS(C)
2A-linked delta-TK
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterTight-TREAvailabilityAcademic Institutions and Nonprofits only -
pDule-pCNF
Plasmid#85494PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)AvailabilityAcademic Institutions and Nonprofits only -
pRAN357
Plasmid#120898PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target proteinDepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailabilityAcademic Institutions and Nonprofits only -
pMflT-o1
Plasmid#101309PurposeContains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMflT-o4
Plasmid#101312PurposeContains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMflCT-o4
Plasmid#101313PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMflET-o4
Plasmid#101314PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
ereB resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMflPT-o4
Plasmid#101315PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
pac resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMflST-o4
Plasmid#101316PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
aadA1 resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9
Plasmid#129391PurposeDerived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.DepositorInsertBurkholderia cenocepacia codon-optimized dCas9
UseCRISPRTagsExpressionBacterialMutationEntire dCas9 codon optimized for the GC-rich B. c…PromoterPrhaBADAvailabilityAcademic Institutions and Nonprofits only -
pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST
Plasmid#175017PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertOK-HEFwtED
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST
Plasmid#175018PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertOK-HEF s57a ED
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutations57a esterase knock out mutantPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST
Plasmid#175019PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertD/bovine/Nebraska/9-5/2012
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1061 - 1080 of 1302 results