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Plasmid#80107PurposePH(R40A)-4GS-I3-01-myc-Late1DepositorInsertEPN-18 (R40A)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔI)
Plasmid#80048PurposeExpression of EPN-01 assembly mutant L33RDepositorInsertEPN-01(L33R)
UseTagsMycExpressionMammalianMutationL33RPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPJ169(JPUB_006769)
Plasmid#83835PurposeCarries NEW operon 5 finalDepositorInsertsKuste3340
Kuste3350
UseTagsExpressionMutationPromoterop5_3350_for and op5_BsaI_3340_forAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPJ170(JPUB_006771)
Plasmid#83833PurposeCarries NEW operon 6 finalDepositorInsertsKuste3346
Kuste3348
Kuste3349
UseTagsExpressionMutationPromoterop6_3348_for, op6_3349_for, and op6_BsmBI_3346_fo…Available sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPJ168(JPUB_006767)
Plasmid#83832PurposeCarries NEW operon 4 finalDepositorInsertsKuste2802
Kuste2805
UseTagsExpressionMutationPromoterop4_2805_for and op4_BsaI_2802_forAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_GB
Plasmid#78677PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_FB
Plasmid#78676PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_EB
Plasmid#78675PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_AB
Plasmid#78674PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWK
Plasmid#62713PurposeDonor vector for amiR-eGFP knock-in via landing padDepositorInsertamiR-eGFP123/amiR-eGFP419/FLPe
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pARA (pKD-G1)
Plasmid#62680PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInserttdTomato/amiR-eGFP123
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pARB (pKD-G2)
Plasmid#62681PurposeMammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancerDepositorInserttdTomato/amiR-eGFP419
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pARD (pKD-G4)
Plasmid#62683PurposeMammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP419
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pATJ (pKD-G8)
Plasmid#62687PurposeMammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP419/amiR-eGFP123
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119
Plasmid#66136PurposeExpresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(T214V)
Plasmid#66137PurposeExpresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationT214VPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-SupH
Plasmid#59710PurposepSALECT with SupH insertDepositorInsertSupH
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-AhyI
Plasmid#59708PurposepSALECT with AhyI insertDepositorInsertAhyI
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST myc Rab35 CA
Plasmid#58382Purposeexpression of drosophila myc-Rab35 constitutively active mutantDepositorInsertRab35 (Rab35 Fly)
UseTagsmycExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab35 CA
Plasmid#58381Purposeexpression of drosophila YFP-Rab35 constitutively active mutantDepositorInsertRab35 (Rab35 Fly)
UseTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 RNAiR2 short
Plasmid#51596Purposeexpresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)DepositorInsertRem2 (Rem2 Rat)
UseTagsmycExpressionMammalianMutationsilent mutations at shRNA 1 and 2 recognition sit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xFNLDD/pMXs-I2
Plasmid#48880PurposeExpresses 3xFNLDD in mammalian cellsDepositorInsert3xFNLDD
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationPromoterLTRAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
XP31 Vox
Plasmid#16972DepositorInsertVox (ventx2.1.L Frog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
WT Ecm2
Plasmid#169922PurposeEcm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416DepositorInsertEcm2
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCDH-NLS-copGFP-EF1-BlastiS
Plasmid#132772PurposeSpills into the cytoplasm during nuclear envelope rupture.DepositorInsertNLS-copGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 mRuby
Plasmid#216225PurposeTDP-43 with a C-terminal mRuby tag for expression in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseTagsmRubyExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA
Plasmid#37120PurposeDO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cellsDepositorInsertTdTomato-EGFP
UseAAV and Cre/Lox; Cre-switchTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucSM EGFP
Plasmid#90171PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
sortase A pentamutant (eSrtA) in pET29
Plasmid#75144Purposeevolved sortase A (eSrtA) pentamutant with improved kinetics and activityDepositorInsertsortase A pentamutant
UseTags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196T. Deleted amino a…PromoterT7Available sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-8xHis-Ub
Plasmid#107392PurposeMammalian expression 8xHis-UbDepositorInsert8 tandem repeats of 6xHis-Ub (See comments below)
UseTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-RFP-shCntrl
Plasmid#69040PurposeLentiviral expression of control shRNADepositorInsertcontrol shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-PIP-FUCCI
Plasmid#138714PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterhPGKAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-EBOV-GP
Plasmid#215692PurposeProduces the Ebola virus glycoproteinDepositorInsertEBOV-GP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Blast-PIP-FUCCI
Plasmid#138715PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-FUCCI
Plasmid#118616PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterhPGKAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHAC-mt-mKeima
Plasmid#72342Purposeretrovirus construct for stable expressing mt-mKeimaDepositorInsertmt-mKeima
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Lime
Plasmid#191255PurposeBacterial expression plasmids for pH sensitive fluorescent protein LimeDepositorInsertLime
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-8XMS2
Plasmid#121939PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG2IG-Hmga1a
Plasmid#122293PurposeOverexpression for mouse Hmga1aDepositorInsertHmga1a (Hmga1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only