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Plasmid#74493PurposeHuman codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNADepositorInsertCas9
UseTagst2a mCherryExpressionMammalianMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Degron-KI ezh2 donor vector
Plasmid#65620PurposeDonor vector to generate Degron-KI DD-EZH2DepositorInsertEZH2 left and right HR arms
UseTagsExpressionMutationPromoterAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB219
Plasmid#61501PurposeConst. Halotag-GFP-PACT + mCherry-DHFRDepositorInsertsHaloTag-GFP-PACT
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-SpHis5
Plasmid#44845PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selectionDepositorInsertyomKO2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomWasabi-Kan
Plasmid#44945PurposeYeast tagging vector to create gene fusion with yeast optimized mWasabi with Kan selectionDepositorInsertyomWasabi
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~P70a-Bottom-T
Plasmid#87314PurposeExpresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.DepositorInsertSplit-Broccoli system
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
PET-3a-chpBK
Plasmid#155201PurposeTo express recombinant ChpBK proteinDepositorInsertschpS-chpBK-6×His-Tag
GyrA-CBD-10xHis-Tag
UseTags10xHis and 6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDHt-SK
Plasmid#63615PurposeThe multiple cloning site of pGreenII0000 cloned into binary vector pDHtDepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B-targeted Aurora B FRET sensor
Plasmid#45232DepositorInsertCENP-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromotert7Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Spa-1 in pCCL-c-MNDU3c-(X2)-pgk-EGFP
Plasmid#38154DepositorInsertsUseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTSTEP-SNAPf
Plasmid#72335PurposeTissue-specifically swappable (C-terminal TagRFPT-to-SNAPf) genome tagging plasmid with Golic+ selection cassette, for fully transgenic gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[Golic+] RRS-SNAPf
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBD21_033
Plasmid#109150PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of SiriusDepositorInsertsmRFP
Sirius
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_036
Plasmid#109153PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2DepositorInsertsmRFP
mKO2
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_034
Plasmid#109151PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFPDepositorInsertsmRFP
TagBFP
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB038b
Plasmid#99212PurposeAccessory plasmid for PACE of PylRS variantsDepositorInsertPpsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
UseTagsExpressionBacterialMutationamber codons at positions 29, 83, and 184 of gIIIPromoterPpsp and PproKAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
XW99
Plasmid#65842PurposeMosSCI-Pmig-13::QS::SL2::mCherry for ttTi4348 on Chrom I.DepositorInsertNeurospora crassa OR74A repressor (qa-1S)
UseTagsmCherryExpressionWormMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomApple-SpHis5
Plasmid#44844PurposeYeast tagging vector to create gene fusion with yeast optimized mApple with SpHis5 selectionDepositorInsertyomApple
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
1025
Plasmid#160235PurposeFlightN-TetR(OFF)-VP16-Sv40-attBDepositorInsertsTetracycline repressor
Flightin promoter
UseTagsVP16ExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenLox_U6 Nlgn1
Plasmid#59339PurposeLentiviral expression vector: Neuroligin 1 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.DepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterCAG and mouse U6Available sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-2
Plasmid#59945Purposedihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humanDepositorInsertsdihydropterine reductase
pterin-4a-carbinolamine dehydratase
UseTagsExpressionMutationPromoterpTrcAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only