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Plasmid#89180PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only
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pScarlessHD-sfGFP-DsRed
Plasmid#80811PurposeVector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsertsfGFP-piggyBac-DsRed
UseUnspecifiedTagssfGFPExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH-Halo tag
Plasmid#154144PurposeGateway compatible mammalian expression plasmid for halo tag fusion proteinsDepositorTypeEmpty backboneUseTagsHalo tagExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPOCK2_22-424_AviN_HisC
Plasmid#195877PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertSPOCK2 (SPOCK2 Human)
UsePfhmsp-avin-lic-c, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-TAF15
Plasmid#181713PurposeGalactose inducible expression of TAF15DepositorInsertTAF15 (TAF15 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
OpenCRISPR-1
Plasmid#221565PurposeExpress OpenCRISPR-1 nuclease in human cells using a CMV promoterDepositorInsertOpenCRISPR-1
UseTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP-AAV-syn-iGABASnFR2n-WPRE
Plasmid#218875PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAVTagsExpressionMammalianMutationS99A F104H R168PPromoterSynapsinAvailable sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInt_attP1_sacB_kanR
Plasmid#205301PurposeIntegrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.DepositorArticleTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXR003_puroR
Plasmid#219819PurposehU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)
Plasmid#175571PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)
Plasmid#48307DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
BirA(R118G)-HA destination vector
Plasmid#53581PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)DepositorTypeEmpty backboneUseTagsBirA(R118G) and HA tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_H2BGFP_WPRE
Plasmid#91788PurposeConstitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.DepositorInsertHuman histone H2B (H2BC11 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-GFP
Plasmid#97030PurposeHis6-GFP-tev-yORFDepositorTypeEmpty backboneUseTagsGFP fusion, His6 tag, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro
Plasmid#192664Purpose3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance markerDepositorInsertdCas9-KRAB-MeCP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, D839A, H840A and N863A in Cas9PromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) FMC-63 scFv-Fc (huFc)
Plasmid#183252PurposeSoluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.DepositorInsertanti-CD19 scFvFc (FMC-63)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF9b
Plasmid#169396PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF9bwith N-term eGFPDepositorInsertSARS-COV-2 _ORF9b
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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USP2
Plasmid#36894PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP2 (USP2 Human)
UseTagsHis tag with thrombin cleavageExpressionBacterialMutationPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits