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Plasmid#75089Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorInsertTTF-1 (NKX2-1 Human)
UseRetroviralTagsnoneExpressionMammalianMutationwild-type, full-length, without native 3' UTRPromoterAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-PQRv3-RFP
Plasmid#73951PurposeProtein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.DepositorInsertssfGFP (superfolder GFP)
RFP
UseTagsA residual PQR peptide wii be left at the C-termi…ExpressionMammalianMutationPromoterChicken beta actin and Chicken beta actin (shared…Available sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EZH1_3
Plasmid#36361DepositorInsertEZH1 (EZH1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX C_K nsp1 CoV2
Plasmid#175516PurposeExpresses C_K for bioconjugation on nsp1DepositorInsertC_K nsp1 CoV2
UseTagsGST tagExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
keratin 8 (krt8)-H2B-RFP
Plasmid#105955PurposetransgenesisDepositorInsertkrt8 (KRT8 Human)
UseTagsRFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-OVOL1-T2A-PuroR
Plasmid#162347PurposeLentiviral expression of OVOL1 under the control of the TetON promoterDepositorInsertOVOL1 (OVOL1 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJP-CoV2leader-nLuc-TSS
Plasmid#175430PurposeExpresses nLuc with 5' leader of CoV2 in mammalian cells.DepositorInsertnLUC plus 5' CoV2 leader
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS
Plasmid#175006PurposeAkt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.DepositorInsertmCherry-Akt-STOPS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
FCK-ArchT-GFP
Plasmid#31177PurposeLentiviral expression of ArchT-GFP for neuronal inhibitionDepositorInsertArchT-GFP
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT006
Plasmid#182716PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloningDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsGFP in cloning site of crRNA for easier screening…ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p164 Dync1i1.B
Plasmid#26435DepositorInsertcytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)
UseSubcloning and sequencingTagsExpressionMutationN/APromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G2-RacE
Plasmid#188966PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
racE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-SLC35B2-sgRNA
Plasmid#154860PurposeLentiviral expression of Cas9 and sgRNA targeting SLC35B2DepositorInsertSLC35B2 sgRNA (SLC35B2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-gp78/JM20
Plasmid#13303DepositorInsertgp78 (AMFR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBS ColIII alpha1
Plasmid#19187DepositorInsertProcollagen type III alpha 1 (COL3A1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-H2B-6
Plasmid#55056PurposeLocalization: Nucleus/Histones, Excitation: 587, Emission: 610DepositorInsertH2B (H2BC11 Human)
UseTagsmCherryExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag HBx (G124L, I127A)
Plasmid#42598DepositorInsertHepatitis B virus protein X (G124L, I127A)
UseTagsFlagExpressionMammalianMutationG124L I127APromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV LacZ L5-L2
Plasmid#62166PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and LacZ module. Compatible with MultiSite Gateway cloningDepositorInsertLacZ
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5B (Kmt5b Mouse)
UseTagsHA-tagExpressionMutationPromoterT3Available sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only