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Plasmid#209863PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddRFP A
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-TurboID-NES_pLX304
Plasmid#202019Purposeexpresses GFP-TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-GFP-TurboID-NES
UseLentiviralTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-H-Luc_cyto
Plasmid#113929PurposePlasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cellsDepositorInsertH-Luc
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-eGFP
Plasmid#176861PurposeExpresses cytosolic eGFP in astrocytesDepositorInserteGFP
UseAAVTagsExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)
Plasmid#159853PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mNeon(PATC)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_221
Plasmid#91115PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCoofy55
Plasmid#122007PurposeMammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis10-SumoStar (Life Sensors) and Multi2: dependi…ExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-miniTurbo-V5_pCDNA3
Plasmid#107174Purposeexpresses V5-tagged miniTurbo on the mammalian ER membraneDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3
Plasmid#209727PurposeExpresses cytosolic medium affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95K.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
APEX2-V5-G3BP1_pTRE-G418
Plasmid#202013Purposeexpresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertAPEX2-V5-G3BP1
UseLentiviralTagsExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo6
Plasmid#203957PurposeCloning plasmid containing the kanamycin resistance gene insertDepositorInsertKanamycin Resistance Gene
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
epi-BE4max-NG
Plasmid#135977PurposeExpresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDawn-sfGFP
Plasmid#107741PurposeExpresses superfolder GFP under the control of a blue-light transcriptional regulatorDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterpLambdaAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-EGFP
Plasmid#162568PurposeUsed as a measure of cPLA2 activation in live cells with an EGFP tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hfYFP
Plasmid#186523PurposeHyperfolder YFP fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertHyperfolder YFP
UseTagsExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN227
Plasmid#127222PurposeBeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDSDepositorInsertCas9, WUS2, AtSTM, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pc DNA FRT TO- APP695
Plasmid#114193PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)DepositorInsertAmyloid-Precursor-Protein (APP Human)
UseTagsExpressionMammalianMutationwild type splice variant 695: 695AA (2085bp, lack…PromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM114
Plasmid#127211PurposeBeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDSDepositorInsertWUS2, IPT, sgRNA, Luc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
Plasmid#209726PurposeExpresses cytosolic medium affinity ratiometric ATP sensorDepositorInsertplenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PM-RA
Plasmid#209878PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43DepositorInsertddRFP A
UseTagsTargeting domain of Gap43 MLCCMRRTKQVEKNDDQKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only