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SEPT11 sh2 shRNA

Plasmid

#122023

Purpose

Knock Down of Septin 11. It targets Septin 11 shRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75212

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pY128

Plasmid

#127713

Purpose

Level 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-LplA(AILR,36)-NRX3b D137A

Plasmid

#43920

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

AILR-LplA36-NRX3b (lplA Human, Synthetic)

Use

Tags

2XHA

Expression

Mammalian

Mutation

D137A

Promoter

Chicken Beta Actin

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pSCKtheoRaj12m

Plasmid

#69943

Purpose

pSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

pSCKtheoRaj12m

Use

Synthetic Biology; Psc101 origin of replication

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75202

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB402SC

Plasmid

#224552

Purpose

Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

Depositor

Paul Schulze-Lefert

Article

Lawson et al bioRxiv 2024.07.14.603419

Type

Empty backbone

Use

Tags

Twin-Strep-HA

Expression

Plant

Mutation

Promoter

2 X 35S

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB402SN

Plasmid

#224551

Purpose

Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

Depositor

Paul Schulze-Lefert

Article

Lawson et al bioRxiv 2024.07.14.603419

Type

Empty backbone

Use

Tags

HA-Twin-Strep

Expression

Plant

Mutation

Promoter

2 X 35S

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2861 (pSpy1C-PgyrA_ffluc)

Plasmid

#218511

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Mutation

deletion of Plac_mrfp cassette

Promoter

PgyrA

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3105 (pSpy1C-PxylS2_ffluc)

Plasmid

#218512

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Mutation

deletion of Plac_mrfp cassette

Promoter

PxylS2

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV110

Plasmid

#215340

Purpose

Expresses sfGFP under induction with the Acrylic Acid Marionette Cassette (acuRAM + PAcu-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

PAcu

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV101

Plasmid

#215307

Purpose

Expresses sfGFP under induction with the Vanillic Acid Marionette Cassette (VanRAM + PVanCC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

PVanCC

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

Plasmid

#214989

Purpose

Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

CRISPR

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

Plasmid

#214990

Purpose

Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (Eea1 Mouse)

Use

CRISPR

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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Cav-RA

Plasmid

#209881

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Use

Tags

CAVIN1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo40

Plasmid

#204015

Purpose

Cloning plasmid containing the mCherry insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

mCherry fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo41

Plasmid

#204016

Purpose

Cloning plasmid containing the BFP insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Blue fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo42

Plasmid

#204017

Purpose

Cloning plasmid containing the YFP insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Yellow fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo5

Plasmid

#203956

Purpose

Cloning plasmid containing the chloramphenicol resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Chloramphenicol Resistance Gene

Use

Tags

Expression

Plant

Mutation

Promoter

CAT promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo7

Plasmid

#203958

Purpose

Cloning plasmid containing the ampicillin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ampicillin Resistance Gene

Use

Tags

Expression

Plant

Mutation

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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