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Plasmid#79017PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only
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pREP82xV5_ccdB
Plasmid#79020PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP42xV5_ccdB
Plasmid#79018PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Plekhm1 - C terminal pFastHFTb
Plasmid#73829PurposeExpress human Plekhm1 C-part in insect cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTags6xHis and FlagExpressionInsectMutationcloned C-terminal part of human Plekhm1 (aa 493- …PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plekhm1-N terminal pFastHFTb
Plasmid#73828PurposeExpress human Plekhm1 N-part in insect cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTags6xHis and FlagExpressionInsectMutationcloned N- terminal part of human Plekhm1 (aa 1- 4…PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF3_1
Plasmid#72363PurposeEncodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFPDepositorInsertZNF3 (ZNF3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF3_2
Plasmid#72364PurposeEncodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFPDepositorInsertZNF3 (ZNF3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_NDUFA6_3UTR
Plasmid#65863PurposeCarries 3' UTR of NDUFA6, an RBPMS targetDepositorInsertNDUFA6 (NDUFA6 Human)
UseTagsExpressionMutationPromoterT7Available sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
psicheck_200_300_CA_repeat
Plasmid#65880PurposeLuciferase assay vector, assessing regulation of 200-300 nucleotide CA repeatsDepositorInsertCA repeats 200-300 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
psicheck_300_400_CA_repeat
Plasmid#65879PurposeLuciferase assay vector, assessing regulation of 300-400 nucleotide CA repeatsDepositorInsertCA repeats 300-400 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
psicheck_50_100_CA_repeat
Plasmid#65882PurposeLuciferase assay vector, assessing regulation of 50-100 nucleotide CA repeatsDepositorInsertCA repeats 50-100 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
4QOX
Plasmid#61959PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPP-CDPK4
UseBaculovirus expressionTagsMHHHHHHSSGRENLYFQGExpressionMutationSee commentsPromoterPolyhedrinAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
4WJC
Plasmid#64655PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPVX_084705:MAC04G-C11:C228845
UseBaculovirus expressionTags6xHisExpressionMutationSee commentsPromoterPolyhedrinAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
3C5I
Plasmid#60118PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPKN-PF14_0020:MAC029-F01:C34272
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
2QQ8
Plasmid#60110PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTBC1D14:TBC1D14_dml-X01:C201000
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
4JUY
Plasmid#60083PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRNF31:YTC010-F07:C227920
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
m2ap
Plasmid#50051Purposeexpresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cellsDepositorInsertM2AP
UseTagsHISExpressionMammalianMutationstarts at aa47; N127R, S158A, S177N, N357S, N463S…PromoterCMVAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
3EOZ
Plasmid#51349PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-PGAM2
UseTags6xHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityAcademic Institutions and Nonprofits only