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Addgene

m2ap
(Plasmid #50051)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50051 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6200
  • Modifications to backbone
    The vector is modified for LIC cloning, but the insert can also be extracted using 5' Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    M2AP
  • Alt name
    MIC2 associated protein
  • Species
    toxoplasma gondii
  • Insert Size (bp)
    900
  • Mutation
    starts at aa47; N127R, S158A, S177N, N357S, N463S, N470D
  • GenBank ID
    AAK51546.1
  • Promoter CMV
  • Tag / Fusion Protein
    • HIS (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Vern B. Carruthers University of Michigan Medical School

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The first 46aa consist of a pro-peptide that gets cleaved during maturation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    m2ap was a gift from Timothy Springer (Addgene plasmid # 50051 ; http://n2t.net/addgene:50051 ; RRID:Addgene_50051)