m2ap
(Plasmid
#50051)
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Purposeexpresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50051 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRES2-EGFP
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6200
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Modifications to backboneThe vector is modified for LIC cloning, but the insert can also be extracted using 5' Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II.
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameM2AP
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Alt nameMIC2 associated protein
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Speciestoxoplasma gondii
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Insert Size (bp)900
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Mutationstarts at aa47; N127R, S158A, S177N, N357S, N463S, N470D
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GenBank IDAAK51546.1
- Promoter CMV
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Tag
/ Fusion Protein
- HIS (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Vern B. Carruthers University of Michigan Medical School
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The first 46aa consist of a pro-peptide that gets cleaved during maturation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
m2ap was a gift from Timothy Springer (Addgene plasmid # 50051 ; http://n2t.net/addgene:50051 ; RRID:Addgene_50051)