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pLVX neo ACTN1-GFP, Y246E

Plasmid

#138294

Purpose

Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP, Y246E fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

ACTN1 (ACTN1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Y246E

Promoter

CMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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IX_pAAV-ProB3-CatCh-GFP-WPRE

Plasmid

#125923

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProB3

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV mouse Vav1 IRES GFP

Plasmid

#107091

Purpose

Mammalian expression retroviral vector

Depositor

Martine Roussel, Steven Clifford

Article

Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

Plasmid

#205152

Purpose

Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

FRT-GFP-FRT-uORF-dCBEevoCDA1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAST

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP-VP16

Plasmid

#183923

Purpose

VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-VP16

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172668

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

ALDOB pegRNA and ALDOB_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET11-His6-Pif6-sfGFP-ssrA

Plasmid

#140891

Purpose

Expression of PIF-sfGFP-ssrA in E. coli

Depositor

Neal Devaraj

Article

Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

PIF-sfGFP-ssrA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Opi1 + terminator

Plasmid

#58836

Purpose

Expresses GFP-Tagged Opi1 + Terminator in yeast

Depositor

Tim Levine

Insert

Opi1p (OPI1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pBa.GFP-KIF3B tail 391-747

Plasmid

#134615

Purpose

Mammalian expression of mouse KIF3B 391-747

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3B (Kif5b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 390 N-terminal residues.

Promoter

Chicken Beta actin

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-223-PGK-GFP

Plasmid

#11378

Depositor

Chang-Zheng Chen

Article

Chen et al Science. 2004 Jan 2. 303(5654):83-6.

Insert

miR-223

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-GFP-MyD88-NpLxIS

Plasmid

#131347

Purpose

Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its N-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88-NpLxIS (Myd88 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

Plasmid

#159283

Purpose

A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Inserts

Human INS sgRNA
Human INS guide RNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

Plasmid

#184335

Purpose

Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

Nrxn3-alpha-GPI (Nrxn3 Mouse)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL1-G20TM

Plasmid

#109019

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL1-deltaTM (ATL1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMKO.1 GFP B56 gamma3 shRNA 14-1

Plasmid

#10681

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma3 shRNA (PPP2R5C Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-FLEX-Mac-GFP

Plasmid

#58852

Purpose

AAV-mediated expression of Mac-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Syn

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-TVA-eGFP-N2cG

Plasmid

#175440

Purpose

Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-hSyn-IntCCCre-T2A-CopGFP

Plasmid

#160503

Purpose

Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

IntCCCre

Use

Cre/Lox and Lentiviral

Tags

CopGFP

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

March 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-I-Sce1-slc6a3[11kb]:egfp

Plasmid

#53240

Purpose

allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

Depositor

Edward Burton

Article

Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

Insert

DAT-GFP

Use

Cloning

Tags

GFP

Expression

Mutation

Promoter

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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Syn:eGFP-CaM-uTEV1Δ(220-242)

Plasmid

#135463

Purpose

Neuronal expression of uFLARE protease component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

eGFP-CaM-uTEV1Δ

Use

AAV

Tags

eGFP, V5

Expression

Mammalian

Mutation

S219V mutation improves stability and S153N impro…

Promoter

Synapsin

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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