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Plasmid#120551PurposeExpress Cas9, 2xNES and 2 tandem ERT2 in mammalian cellsDepositorInsertCas9, NES and ERT2
UseLentiviralTagsExpressionMammalianMutationPromoterEF1αAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64
Plasmid#203350PurposeTemplate for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertNano Luciferase
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:ELuc
Plasmid#118062PurposeTranscriptional unit for constitutive expression of ELuc in mammalian cellsDepositorInsertCMV:ELuc:bGH
UseLuciferase and Synthetic Biology; Assembly of gb2…TagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-CHMP4B
Plasmid#139013PurposeExpresses HA-tagged human CHMP4B in mammalian cellsDepositorInserthuman CHMP4B
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU-Gm23
Plasmid#53738PurposeUsed for in vitro transcription/translation of soybean His-tagged arginyl-tRNA synthetase in the Cell Free Sciences systemDepositorInsertarginyl-tRNA synthetase
UseSynthetic BiologyTagsHis tagExpressionMutationPromoterSP6Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR
Plasmid#171382PurposeExpression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized dCas13X.1-REPAIRv2
UseTagsHAExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycYBX1
Plasmid#19878DepositorInsertY box binding protein 1 (YBX1 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3196
Plasmid#172696PurposePrgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)DepositorInsertPrgef-1
UseTagsGFP 1-10ExpressionWormMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
SGEP
Plasmid#111170PurposemiR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterSFFVAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Tet-UPF1WT (pRKB264)
Plasmid#99146PurposeInducible lentiviral construct to express FLAG-tagged UPF1wtDepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiSAM v2 (Puro)
Plasmid#92062PurposeModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 (sgRNA) and EF1a (dCas9-VP64)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-Claspin
Plasmid#12659DepositorInsertClaspin (CLSPN Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pLBS-EF-QUE37C
Plasmid#129338PurposeATP measurement, lentivirusDepositorInsertQUEEN-37C
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cys-30nmSAH-SPYcatcher
Plasmid#214839PurposeThis plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-30nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hsyn-GRAB_5-HT1.0
Plasmid#140552PurposeExpresses the 5-HT sensor GRAB_5-HT1.0 in neuronsDepositorHas ServiceAAV9Insert5-HT sensor GRAB_5-HT1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A-FBL/PITCh
Plasmid#63671PurposeExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG030 (TerY-P)
Plasmid#157908PurposeExpresses the TerY-P defense systemDepositorInsertTerY-P triad (vWA + kinase + phosphatase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
4xCSL-luciferase
Plasmid#41726DepositorInsert4xCSL binding sites
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains a multimerized high affinity CSL binding…PromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only