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Plasmid#118387PurposeEntry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertdCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYC_HA-ZmSAE1_ZmSAE2-FLAG
Plasmid#91944PurposeBacterial expression of maize SAE1 and SAE2DepositorInsertsHA-ZmSAE1
ZmSAE2-FLAG
UseTagsFLAG and HAExpressionBacterialMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL1-GFP
Plasmid#180420PurposeKan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2ST
Plasmid#59384PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.DepositorInsertTT-ISceI-dfrA
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Launchpad (AVA2590)
Plasmid#85442PurposeLentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassetteDepositorInsertFRT-flanked EF1alfa-GFP PGK-Puromycin cassette
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK105
Plasmid#59562PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PESTDepositorInsertSGP(14) 2xK-turn EYFP-PEST
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBLO1811_Cas9_noNLS_human
Plasmid#74490PurposeHuman codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNADepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGTag Hygro EGFP nLAP +0
Plasmid#194305PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertHygro-P2A-nLAP(EGFP)
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ2007
Plasmid#204399PurposeA 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsquorum sensing regulator LuxR
GFP
UseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PLux0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ4004
Plasmid#204401PurposeA 3OHC14-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsmCinR
GFP
UseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PCin0102DAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM710
Plasmid#68893PurposeIPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
UseTagsExpressionMutationPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERB215
Plasmid#61499PurposeConst. Halotag-GFP-Nuf2 + mCherry-DHFRDepositorInsertsHaloTag-GFP-Nuf2
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP-T-CaURA3
Plasmid#44877PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP-T with CaUra3 selectionDepositorInsertyoTagRFP-T
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
XW52
Plasmid#65838PurposeXW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional methodDepositorInsertCzipper-QF-AD-NLS
UseTagsmCherryExpressionWormMutationPromoterPmig-13Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKeima-Kan
Plasmid#44902PurposeYeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selectionDepositorInsertyomKeima
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDB007ns2a
Plasmid#99214PurposeAccessory plasmid for PACE of PylRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
UseTagsExpressionMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PtetAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRB_p2_TCRA
Plasmid#131034PurposeExpresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptideDepositorArticleUseTagsExpressionMammalianMutationPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
LZF40: hSyn-Cre
Plasmid#104124PurposesynOptopatch constructDepositorInsertCre
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only