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Plasmid#164267PurposesgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting the coding sequence of dpy-10
UseCRISPRTagsExpressionWormMutationPromoterR07E5.16 (U6)Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF495_dpy-10_CDS_sg6
Plasmid#164268PurposesgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.DepositorInsertsgRNA targeting the coding sequence of dpy-10
UseCRISPRTagsExpressionWormMutationPromoterU6 promoter from W05B2.8Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB ONLY R3
Plasmid#161511PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R3_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting R3 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB ONLY R4
Plasmid#161512PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R4_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting R4 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T1
Plasmid#161498PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T1 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T2
Plasmid#161499PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T2_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T2 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T3
Plasmid#161500PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T3_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T3 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T4
Plasmid#161501PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T4_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T4 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pALD4 T5
Plasmid#161502PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T5 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T-
Plasmid#161503PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Non targeting control NTC)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T1
Plasmid#161504PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T1 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T2
Plasmid#161505PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T2_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T2 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T3
Plasmid#161506PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T3_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T3 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T4
Plasmid#161507PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T4_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T4 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI ONLY pGAP T5
Plasmid#161508PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting T5 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB ONLY R1
Plasmid#161509PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R1_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting R1 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB ONLY R2
Plasmid#161510PurposeNon activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R2_gRNA_MS2DepositorInsertsdCAS9
MS2 (non activation control NAC)
gRNA (Targeting R2 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T4
Plasmid#161485PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T4_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (targeting T4 of pTHI11 promoter)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T5
Plasmid#161486PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (targeting T5 of pTHI11 promoter)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T2
Plasmid#161489PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T2_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T2 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only