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Plasmid#113984PurposeVector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertRK2 (IncP)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-S-Luc_cyto
Plasmid#113926PurposePlasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cellsDepositorInsertS-Luc
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)
Plasmid#159853PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mNeon(PATC)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTC223
Plasmid#70019PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7DepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTW102
Plasmid#115945PurposeORF insert for replicon MoCloDepositorInsertLvl0 DDd-L7Ae
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAV0620
Plasmid#133506PurposeExpression of eGFP tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_HaloK73T_cyto
Plasmid#183687PurposeMammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)DepositorInsertCyto Halo(K73T)
UseTagsExpressionMammalianMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2359 - intron GG2 - 250bp
Plasmid#159878PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG2 - 250bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2369 - intron GG1 - 250bp
Plasmid#159877PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG1 - 250bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase
Plasmid#25846DepositorInsertLplA-W37I
UseTagsFlag and HisExpressionMammalianMutationW37IPromoterAvailable sinceJuly 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-riboB
Plasmid#168975PurposeFilamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboBDepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus nidu…TagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-H13LTat-CD8a/d2eGFP-IRES-Nef
Plasmid#126552PurposeReplication incompetent HIV with H13L Tat and d2eGFP/CD8a reporterDepositorInsertHIV-1 vector pNL4-3
UseLentiviralTagsCD8a and GFPExpressionMutationDelta gag/polPromoterHIV LTRAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
CJ8421_04975 Rptr2 and CJ8421_04975 mRNA
Plasmid#194210PurposeExpresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2DepositorInsertCJ8421_04975 mRNA and CJ8421_04975 Rptr2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2370 - intron GG3 - 250bp
Plasmid#159879PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG3 - 250bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
epi-ABEmax
Plasmid#135974PurposeExpresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
DCIP
Plasmid#193860PurposeEncodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertNLS-mCherry-GFP1-10
UseTagsSV40 NLSExpressionPlantMutationPromoter35SAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-LEU2
Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-SacB-Cas9-T2A-mCherry_(BbsI)
Plasmid#117070PurposeEncodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selectionDepositorInsertsCas9
SacB
UseCRISPRTags3x FLAG, NLS, and T2A-mCherryExpressionMammalianMutationPromoterCBh and U6Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only