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Plasmid#202601PurposeRP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only
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pNES-iRFP-C1
Plasmid#116863Purposecytosolic iRFPDepositorInsertiRFP713
UseTagsX.leavis map2k1.L(32-44)ExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Halo-Cyto
Plasmid#124314PurposeExpresses cytosolic specific HaloTag proteinDepositorInsertHalo tag
UseTagsCytoplasm specific seqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-DeltaJunD
Plasmid#68547PurposeAAV expression of DeltaJunDDepositorInsertDeltaJunD-IRES-GFP
UseAAVTagsExpressionMammalianMutationtruncated splice variantPromoterCMVAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Clover-Prx2-mRuby2
Plasmid#117907Purposefluorescent reporter of cytosolic hydrogen peroxideDepositorInsertClover-Prx2-mRuby2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-crRNA-Km
Plasmid#158712PurposePlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNADepositorInsertAcGFP (for crRNA cloning)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterTetOAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPJ23116-MS2-rep
Plasmid#184842PurposeExpression of the rep protein of phage MS2DepositorInsertReplicase gene MS2 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23116Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3xhfYFP
Plasmid#186544PurposeHyperfolder YFP, triple fusion: cytosolic expression in mammalian cellsDepositorInsertHyperfolder YFP (3X)
UseTagsExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-H-Luc_cyto
Plasmid#113929PurposePlasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cellsDepositorInsertH-Luc
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_231
Plasmid#91120PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG380
Plasmid#91188PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)DepositorInsertAtCas9+gNt_R2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_211
Plasmid#91110PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cav-GA
Plasmid#209880PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1DepositorInsertddGFP A
UseTagsCAVIN1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP
Plasmid#153008PurposeExpresses glucagon peptide on mammalian cell surfaceDepositorInsertGlucagon-ICAM1-pre-mGRASP
UseTags3x HAExpressionMammalianMutationPromoterCAGAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-StagRFP-EKAR-EV
Plasmid#173019PurposeRed/far-red FRET-based ERK activity reporter for multiplexed imaging applications.DepositorInsertsmURFP-StagRFP-EKAR-EV
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM253 - sgRNA mosTI hygroR
Plasmid#159827PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertsgRNA mosTI hygroR
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef
Plasmid#126553PurposeReplication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporterDepositorInsertHIV-1 vector pNL4-3
UseLentiviralTagsThy1.2 and GFPExpressionMutationPromoterHIV LTRAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM270 - MCS mosTI hygroR target
Plasmid#159826PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertMCS mosTI hygroR target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha1
Plasmid#165862PurposeAlpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPCX roGFP2-Prx2(C172A)
Plasmid#158228PurposeGenetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)DepositorInsertroGFP2-Prx2(C172A)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only