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NK639

Plasmid

#109102

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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NK640

Plasmid

#109103

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_06009

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK642

Plasmid

#109101

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_07215

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK591

Plasmid

#109080

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mTFP1

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK586

Plasmid

#109069

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_05705

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK613

Plasmid

#109091

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry_SV40 NLS

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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NK648

Plasmid

#109104

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

3'UTR EFL

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK597

Plasmid

#109086

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

tdTomato

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK595

Plasmid

#109083

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

EGFP

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK585

Plasmid

#109079

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK609

Plasmid

#109078

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK588

Plasmid

#109077

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

pActAvrII_nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Eliminate NcoI site in the promoter

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK606

Plasmid

#109076

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK587

Plasmid

#109075

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK583

Plasmid

#109072

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_10057

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NK600

Plasmid

#109068

Purpose

Parental vector for making S. rosetta expression vectors

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_02256

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TbSIR2RP1

Plasmid

#67242

Purpose

Determine its activity and specificity

Depositor

Cynthia Wolberger

Article

Fahie et al FEBS J. 2009 Dec;276(23):7159-76. doi: 1

Insert

TbSIR2RP1

Use

Tags

MBP, TEV, Thrombin, HIS, and none

Expression

Bacterial

Mutation

Promoter

p-lac

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pJK600

Plasmid

#71605

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Use

Tags

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, histidine…

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1C10

Plasmid

#70841

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG6907

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2E03

Plasmid

#70880

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG8378

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only