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Plasmid#186643PurposeaadA.co-TaadA-PJ23111-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23111-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH141
Plasmid#186644PurposeaadA.co-TaadA-PJ23100-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23100-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH142
Plasmid#186645PurposeaadA.co-TaadA-PJ23107-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23107-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH143
Plasmid#186646PurposeaadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23117-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBJ 2358
Plasmid#89245PurposeExpresses YFP fused to the N-terminus of Mouse Capping Protein Alpha1, using the Clontech vector PEYFP C-1.DepositorInsertCapza1 (Capza1 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMeCP2Y pc644
Plasmid#167565PurposeThe complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)DepositorInsertMethyl-CpG binding protein 2 (Mecp2 Rat)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I affinity mutant (F40-based tension sensor)
Plasmid#119190PurposeThe affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.DepositorInserthuman Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PPO-Venus
Plasmid#139505PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV8 and AAV9Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3
Plasmid#213969PurposeAAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinaseDepositorInsertShank3 sgRNA
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-PPO-Venus
Plasmid#139504PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV5Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-q-CFP
Plasmid#66081PurposeThis G protein alpha-q construct contains internal insertions of CFP and the EE epitopeDepositorInsertG protein alpha-q-EE-CFP (Gnaq Mouse)
UseTagsCFP was inserted internally into the alpha-q sequ…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
mini-YFP-hDF-C2F-TM
Plasmid#72600Purposemini human dysferlin (from domain F to transmembrane ) tagged with YFPDepositorInsertdysferlin (DYSF Human)
UseTagsEYFPExpressionMammalianMutationfrom domain F to transmembrane domainPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pABD1031
Plasmid#68004PurposeProtein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in plantaDepositorInsertPHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
UseTagseYFPExpressionPlantMutationPromoter2X Cauliflower Mosaic Virus 35S promoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N1 (804)
Plasmid#61854Purposeempty backbone for tagging proteins at the C-terminus with VenusDepositorTypeEmpty backboneUseTagsVenusExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
mVenus N1
Plasmid#27793DepositorHas ServiceCloning Grade DNAInsertmVenus N1
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
mVenus C1
Plasmid#27794DepositorHas ServiceCloning Grade DNAInsertmVenus C1
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
cdk1 FRET sensor (2327)
Plasmid#26064DepositorInsertCdk1 FRET Sensor
UseTagsCFP variant, FLAG, and YFP variantExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
mRuby3-C1
Plasmid#127808PurposemRuby3 C1 Cloning vectorDepositorInsertmRuby3
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
inactive cdk1 FRET sensor (2328)
Plasmid#26065DepositorInsertCdk1 FRET Sensor- Inactive
UseTagsCFP variant, FLAG, and YFP variantExpressionMammalianMutationSer 126 mutated to AlaPromoterAvailable sinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -